安息香不同提取方法的GC-MS研究  

The GC-MS Research on Different Extraction Methods of Benzoinum

作　　者：彭颖[1] 夏厚林[1] 周颖[1] 王建[1] 黄立华[1] 

机构地区：[1]成都中医药大学,成都611137

出　　处：《中国实验方剂学杂志》2012年第19期73-76,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家重点基础研究发展计划(973计划)项目(2007CB512606)

摘　　要：目的:考察安息香不同提取方法的化学成分异同,探索安息香挥发性成分分析的最宜提取方法。方法:应用气相色谱-质谱联用技术,分析安息香溶剂提取部位(石油醚和乙醚提取)、水蒸气蒸馏提取挥发油、顶空固相微萃取挥发性成分的异同。结果:安息香溶剂提取鉴定出5个化合物(其中石油醚部位4个化合物、乙醚部位4个化合物),水蒸气蒸馏提取的挥发油鉴定出19个化合物,顶空固相微萃取鉴定出12个化合物。3种方法所得成分中皆含有苯甲酸、苯甲酸苄酯、香草醛、肉桂酸苄酯4种成分,3种方法中常规水蒸气蒸馏得到的成分相对较多种类较全面。结论:3种提取方法中,安息香经常规水蒸气蒸馏提取得到的成分最多且种类最全面,提示此3种提取方法中常规水蒸气蒸馏是安息香挥发性成分分析的最宜方法。Objective：To identify the differences and similarities of chemical components obtained through different extraction methods about Benzoinum and to explore the best extraction method to study the volatile components of Benzoinum.Method：The similarities and differences among solvent extraction（by petroleum ether and ethyl ether）,extraction of volatile oils through steam distillation and extraction of volatile components through HS-SPME were analyzed and compared by the GC-MS technology.Result： 5 compounds were identified through the extraction of the solvent of Benzoinum,including 4 compounds in the part of petroleum ether and 4 compounds in the part of ethylether.19 compounds were identified during the volatile oils extracted by steam distillation.12 compounds were identified through SPME.The components obtained through three methods all include 4 chemical constitutions such as benzoic acid,benzyl benzoate,vanillin and benzyl cinnamate.The steam distillation obtained the most components in this three extraction methods.Conclusion：The steam distillation obtained the most components in this three extraction methods.It suggested that the steam distillation is the best method to study the volatile components of Benzoinum.

关 键 词：安息香 溶剂提取 水蒸气蒸馏 顶空固相微萃取 气相色谱-质谱联用 

分 类 号：R284.1[医药卫生—中药学]