提高丙酮丁醇梭菌产溶剂稳定性及防止退化的研究  

Studies on enhancing stability of solvent producing capacity of Clostridium acetobutylicum and preventing its degeneration

作　　者：罗玮[1] 周灿灿[1] 潘鹤鹏[1] 李汉广[1] 刘晓波[1] 顾秋亚[1] 余晓斌[1] 

机构地区：[1]江南大学生物工程学院糖化学与生物技术教育部重点实验室,无锡214122

出　　处：《工业微生物》2012年第5期1-6,共6页Industrial Microbiology

基　　金：国家自然科学基金项目(21176105);中央高校基本科研业务费专项资金(JUSRP111A24)

摘　　要：在丙酮丁醇梭菌连续传代过程中,添加乙酸钠可增强其稳定性,同时在未添加乙酸钠的发酵液中分离获得溶剂产量明显降低的退化菌株DNU83,其丁醇产量为2.33 g·L^(-1),仅为初始菌株的1/6。培养基中添加乙酸钠、丁酸钠或K_2HPO_4等弱酸盐均可恢复退化菌株的产溶剂能力,如同时添加苄基紫精,可显著促进丁醇合成。7%玉米培养基中添加4 g·L^(-1) K_2HPO_4和30 mg·L^(-1)苄基紫精,丁醇产量可达18.01 g·L^(-1),总溶剂21.59 g·L^(-1),丁醇比为83.43%,丁醇产量较未退化菌株NU22提高24.09%。The addition of sodium acetate to Meanwhile, a degenerated strain designated medium could stabilize the solvent production during continuous subcultures. as DNU83 with an obvious reduced production of solvent was isolated from the broth without addition of sodium acetate. The butanol production of DNU83 drastically decreased to one-sixth of that of NU22. Supplement of weak-acid salt such as acetate, butyrate and phosphate into medium could restore the solvent production of the degenerated DNU83. When benzyl viologen was simultaneously added, the promoted effect on butanol production was obvious. The results indicated that Clostridium acetobutylicum DNU83 produced 18.01 g . L-1 butanol with a butanol ratio of 83.43% as grew in corn medium containing 30 mg ,L-1 benzyl viologen and 4 g . L-1I K2HPO4. The butanol production was 24.09% higher than that of Clostridium acetobutylicum NU22

关 键 词：丁醇 退化 苄基紫精 乙酸钠 弱酸盐 

分 类 号：TQ923[轻工技术与工程—发酵工程]