β-谷甾醇对脂质体抗氧化性及膜结构稳定性的影响  

Effect of β-sitosterol on antioxidation and stability of liposomes

作　　者：贺然[1] 刘国琴[1,2] 李琳[1,2] 

机构地区：[1]河南工业大学粮油食品学院,郑州450001 [2]华南理工大学轻工与食品学院,广州510640

出　　处：《中国油脂》2012年第9期57-61,共5页China Oils and Fats

基　　金：国家自然科学基金项目(31130042;20976036);国家科技支撑计划项目(2012BAD37B00);广东省科技计划项目(2010B031700014;2010B050600003)

摘　　要：以旋转蒸发-超声法制备脂质体,比较了以β-谷甾醇为助膜材料的脂质体(PC/Sito脂质体)、以胆固醇为助膜材料的脂质体(PC/Chol脂质体)和不加助膜材料的脂质体(PC脂质体)在抵抗超声作用、AAPH(2,2-偶氮二(2-甲基丙基咪)二盐酸盐)引发的氧化作用、紫外线引发的氧化作用过程中的区别。结果表明:相比PC脂质体,PC/Sito脂质体和PC/Chol脂质体具有明显抵抗超声作用和推迟由AAPH引发的脂质体氧化反应发生的作用,并能有效维持脂质体在超声和氧化过程中脂质体的稳定性;在维持脂质体稳定性的效果上,β-谷甾醇优于胆固醇;在抵抗紫外线引发的脂质体氧化的过程中,3种脂质体未表现出明显区别。Liposomes were prepared by conventional rotary evaporation - sonication method from lecithin with β- sitosterol or cholsterol or nothing, forming three different liposome systems, such as PC/Sito lipo- somes, PC/Chol liposomes and PC liposomes. The effects of ultrasonic, AAPH and ultraviolet radiation on each liposomes were evaluated. The results indicated that the PC/Sito liposomes and PC/Chol liposomes could stand against ultrasonic and delay oxidation induced by AAPH compared with PC liposomes. Fur- thermore ,β- sitosterol and cholesterol could maintain the stability of the liposomes through ultrasonic and oxidation induced by AAPH, and β-sitosterol was more effective. There was no obviously difference a- mong the three systems in the oxidation induced by ultraviolet radiation.

关 键 词：脂质体 Β-谷甾醇 AAPH 紫外线 旋转蒸发-超声法 

分 类 号：TQ641[化学工程—精细化工]