反相高效液相色谱法测定石榴籽中鞣花酸的含量  

Determination of Ellagic Acid in Pomegranate Seeds by RP-HPLC

作　　者：刘振平[1] 陈祥贵[1] 彭海燕[1] 杨文宇[1] 何宇新 杨潇[1] 李玉锋[1] 

机构地区：[1]西华大学生物工程学院食品生物技术四川省高校重点实验室,四川成都610039

出　　处：《食品科学》2012年第18期220-222,共3页Food Science

基　　金：国家星火计划项目(2010GA810002);四川省科技攻关项目(05SG011-021)

摘　　要：目的:建立检测石榴籽中鞣花酸的含量测定方法。方法:采用反相高效液相色谱法,Arcus EP-C18(250mm×4.6mm,5μm)色谱柱,以甲醇-0.1%TFA溶液为流动相梯度洗脱,流速1.0mL/min,检测波长254nm。结果:鞣花酸0.004～0.064μg之间线性关系良好,平均加样回收率为100.49%。结论:本方法精密可靠,可作为检测石榴籽中鞣花酸含量的方法。Objective： To establish an HPLC method for the determination of ellagic acid in pomegranate seeds. Methods： The chromatographic separation was carried out on an Arcus EP-C18 column （250 mm× 4.6 mm, 5/μm） using a mobile phase composed of a mixture of methanol and 0.1% TFA at a flow rate of 1.0 mlJmin by gradient elution. The detection wavelength was 254 nm. ResultsThe linear range was 0.004-0.064 μg for ellagic acid. The average recovery rate was 100.49%. Conclusion： This method is accurate, reproducible and applicable for quality control of ellagic acid in pomegranate seeds.

关 键 词：石榴籽 鞣花酸 反相高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]