前肽缺失vWF促进细胞分泌蛋白质剪接的L303E/F309S突变体凝血第八因子  

Propeptide-deleted von Willebrand Factor Improves Secretion of Protein Spliced L303E/F309S Mutated FⅧ

作　　者：朱甫祥[1] 刘泽隆[1] 缪静[1] 屈慧鸽[1] 迟晓艳[1] 

机构地区：[1]鲁东大学生命科学学院,烟台264025

出　　处：《中国细胞生物学学报》2012年第10期988-991,共4页Chinese Journal of Cell Biology

基　　金：山东省自然科学基金(No.ZR2010CM061)资助项目~~

摘　　要：该文旨在探索前肽缺失的von Willebrand因子(vWF-ΔPro)对蛋白质剪接的L303E/F309S突变体凝血第八因子(FVIII)分泌的影响。将vWF-ΔPro基因与蛋白内含子融合的FVIII重链和轻链基因共转HEK293细胞。结果显示,转vWF-ΔPro细胞的剪接蛋白FVIII分泌量和活性分别为(196±27)ng/mL和(1.39±0.31)IU/mL,明显高于对照细胞的(116±24)ng/mL和(0.91±0.18)IU/mL。表明vWF-ΔPro可提高剪接的L303E/F309S突变体FVIII蛋白分泌量和活性。We recently demonstrated that L303E and F309S mutation in the A1 domain of heavy chain of coagulation factor VⅢ （FVⅢ） could improve secretion of spliced FVIII in protein-splicing based dual-vector delivery of FVⅢ gene. In this study, we further investigated the effect of a propeptide-deleted form of the von Wiilebrand factor （vWF-APro）, a functional FVIII carrier co-transfection on secretion of protein spliced FVⅢ with L303E/F309S mutation. By co-transfection of HEK293 cell with both heavy and light chain genes fused to intein, a protein splicing element and vWF-4Pro gene, an ELISA was performed to determine secreted spliced FVⅢand Coatest was used to measure secreted bioactivity. The data demonstrated that vWF-APro co-expressed cell displayed a much higher levels of secretion of spliced FVIII （196±27） ng/mL, compared to control cell （116±24） ng/mL. The secreted bioactivity by vWF-APro co-expressed cell （1.39±0.31） IU/mL was also greater than that of control cell （0.91±0.18） IU/mL. Therefore, vWF-APro may further improve efficacy of dual-vector delivery of FVⅢ gene by enhancing secretion of spliced L303E/F309S mutated FVⅢ encouraging our ongoing in vivo investigation for improvement of two-vector FVIII transgene.

关 键 词：VWF 凝血第八因子 蛋白质剪接 分泌 

分 类 号：R363[医药卫生—病理学]