Effect of Oxidative Stress on Ventricular Arrhythmia in Rabbits with Adriamycin-induced Cardiomyopathy  

作　　者：贺莉 肖建民 付晖 杜广胜 肖幸 张存泰 顾晔 马业新 

机构地区：[1]Department of Cardiology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology [2]Department of Cardiology,Puai Hospital [3]Department of Cardiology,Dongguan Taiping Hospita [4]Department of Pharmacology,Guangdong Medical College

出　　处：《Journal of Huazhong University of Science and Technology(Medical Sciences)》2012年第3期334-339,共6页华中科技大学学报（医学英德文版）

基　　金：supported by grants from the National Natural Science Foundation of China(No.30971180);the Natural Science Foundation of Hubei Province of China(No.2009CDB127)

摘　　要：The purpose of the present study was to examine the effects of oxidative stress on ventricular arrhythmias in rabbits with adriamycin-induced cardiomyopathy and the relationship between oxidative stress and ventricular arrhythmia. Forty Japanese white rabbits were randomly divided into four groups （n=10 in each）： control group, metoprolol （a selective β1 receptor blocker） group, carvedilol （a nonselective β blocker/α-1 blocker） group and adriamycin group. Models of adriamycin-induced car-diomyopathy were established by intravenously injecting adriamycin hydrochloride （1 mg/kg） to rabbits via the auri-edge vein twice a week for 8 weeks in the adriamycin, metoprolol and carvedilol groups. Rabbits in the control group were given equal volume of saline through the auri-edge vein. Rabbits in the metoprolol and carvedilol groups were then intragastrically administrated metoprolol （5 mg/kg/d） and carvedilol （5 mg/kg/d） respectively for 2 months, while those in the adriamycin and control groups were treated with equal volume of saline in the same manner as in the metroprolol and carvedilol groups. Left ventricular end diastolic diameter （LVEDd） and left ventricular ejection fraction （LVEF） were measured by echocardiography. Plasma levels of N-terminal pro B-type natriuretic peptide （NT-proBNP）, malondialdehyde （MAD） and superoxide dismutase （SOD） were detected. The left ventricular wedge preparations were perfused with Tyrode＇s solution. The transmural electrocardiogram, transmural action potentials from epicardium （Epi） and endocardium （Endo）, transmural repolarization dispersion （TDR） were recorded, and the incidences of triggered activity and ventricular arrhythmias were obtained at rapid cycle lengths. The results showed that TDR and the serum MDA and NT-proBNP levels were increased, and LVEF and the serum SOD level decreased in the adriamycin group compared with the control group. The incidences of triggered activity and ventricular arrhythmia were signiThe purpose of the present study was to examine the effects of oxidative stress on ventricular arrhythmias in rabbits with adriamycin-induced cardiomyopathy and the relationship between oxidative stress and ventricular arrhythmia. Forty Japanese white rabbits were randomly divided into four groups （n=10 in each）： control group, metoprolol （a selective β1 receptor blocker） group, carvedilol （a nonselective β blocker/α-1 blocker） group and adriamycin group. Models of adriamycin-induced car-diomyopathy were established by intravenously injecting adriamycin hydrochloride （1 mg/kg） to rabbits via the auri-edge vein twice a week for 8 weeks in the adriamycin, metoprolol and carvedilol groups. Rabbits in the control group were given equal volume of saline through the auri-edge vein. Rabbits in the metoprolol and carvedilol groups were then intragastrically administrated metoprolol （5 mg/kg/d） and carvedilol （5 mg/kg/d） respectively for 2 months, while those in the adriamycin and control groups were treated with equal volume of saline in the same manner as in the metroprolol and carvedilol groups. Left ventricular end diastolic diameter （LVEDd） and left ventricular ejection fraction （LVEF） were measured by echocardiography. Plasma levels of N-terminal pro B-type natriuretic peptide （NT-proBNP）, malondialdehyde （MAD） and superoxide dismutase （SOD） were detected. The left ventricular wedge preparations were perfused with Tyrode＇s solution. The transmural electrocardiogram, transmural action potentials from epicardium （Epi） and endocardium （Endo）, transmural repolarization dispersion （TDR） were recorded, and the incidences of triggered activity and ventricular arrhythmias were obtained at rapid cycle lengths. The results showed that TDR and the serum MDA and NT-proBNP levels were increased, and LVEF and the serum SOD level decreased in the adriamycin group compared with the control group. The incidences of triggered activity and ventricular arrhythmia were signi

关 键 词：heart failure CARVEDILOL oxidative stress ADRIAMYCIN ventricular arrhythmias delayed af-terdepolarizations 

分 类 号：R541.7[医药卫生—心血管疾病]