Inhibitory Effects of MicroRNA-34a on Cell Migration and Invasion of Invasive Urothelial Bladder Carcinoma by Targeting Notch1  

作　　者：张超 姚志勇 朱鸣阳 马鑫 史涛坪 李宏召 王保军 欧阳金芝 张旭 

机构地区：[1]Department of Urology, Chinese PLA General Hospital [2]Nankai University [3]Department of Urology, Air Force General Hospital of PLA [4]Department of Endocrinology, Chinese PLA General Hospital

出　　处：《Journal of Huazhong University of Science and Technology(Medical Sciences)》2012年第3期375-382,共8页华中科技大学学报（医学英德文版）

基　　金：supported by a grant from the National Natural Science Foundation of China(No.30972982)

摘　　要：MicroRNAs （miRNAs or miRs） are a class of short, non-coding RNAs that participate in various oncological processes. This study aims to explore the roles of microRNA-34a （miR-34a） in invasive urothelial bladder carcinoma. miR-34a was transfected into bladder cancer cell lines 253J and J82. The miR-34a expression levels in tissues and cells were detected by using qRT-PCR. The Notch1 expression was detected by qRT-PCR and Western blotting. Cell migratory and invasive abilities were measured by Transwell chamber assay. Bioinformatics and luciferase assay were performed to predict and analyze the binding sites between miRNA-34a and Notch1. It was found that there was aberrant expression of miR-34a in bladder cancer tissues. Moreover, we revealed that ectopic expression of miR-34a suppressed cell migration and invasion, while forced expression of Notch1 increased cell migratory and invasive abilities. Finally, we observed that miR-34a transfection significantly down-regulated luciferase activity and reduced the mRNA and protein levels of Notch1. Our study concluded that microRNA-34a antagonizes Notch1 and inhibits cell migration and invasion of bladder cancer cells, which indicates the tumor-suppressive function of microRNA-34a in bladder cancer.MicroRNAs （miRNAs or miRs） are a class of short, non-coding RNAs that participate in various oncological processes. This study aims to explore the roles of microRNA-34a （miR-34a） in invasive urothelial bladder carcinoma. miR-34a was transfected into bladder cancer cell lines 253J and J82. The miR-34a expression levels in tissues and cells were detected by using qRT-PCR. The Notch1 expression was detected by qRT-PCR and Western blotting. Cell migratory and invasive abilities were measured by Transwell chamber assay. Bioinformatics and luciferase assay were performed to predict and analyze the binding sites between miRNA-34a and Notch1. It was found that there was aberrant expression of miR-34a in bladder cancer tissues. Moreover, we revealed that ectopic expression of miR-34a suppressed cell migration and invasion, while forced expression of Notch1 increased cell migratory and invasive abilities. Finally, we observed that miR-34a transfection significantly down-regulated luciferase activity and reduced the mRNA and protein levels of Notch1. Our study concluded that microRNA-34a antagonizes Notch1 and inhibits cell migration and invasion of bladder cancer cells, which indicates the tumor-suppressive function of microRNA-34a in bladder cancer.

关 键 词：microRNA-34a urothelial carcinoma migration INVASION NOTCH1 

分 类 号：R737.14[医药卫生—肿瘤]