应用Fluoro-Jade C评价匹罗卡品模型中新大脑皮质神经变性  

Assessment of neurodegeneration in neocortex of pilocarpine-treated mice by Fluoro-Jade C staining

作　　者：王莲[1] 魏玲[1] 付莉[1] 

机构地区：[1]成都军区昆明总医院地干病房,昆明650032

出　　处：《西南国防医药》2012年第10期1048-1050,共3页Medical Journal of National Defending Forces in Southwest China

摘　　要：目的应用Fluoro-Jade C(FJC)在小鼠匹罗卡品癫痫模型中探测新大脑皮质结构中神经元的变性情况。方法雄性昆明种小鼠10只(对照组5只,匹罗卡品处理组5只)。对照组给予阿托品、生理盐水腹腔注射;匹罗卡品处理组给予阿托品、匹罗卡品腹腔注射诱发癫痫持续状态。在癫痫持续状态后12 h,通过经心灌注固定处死匹罗卡品处理组小鼠。对照组小鼠处死时间及方法同匹罗卡品处理组。在各新大脑皮质水平切制冠状切片,行FJC染色,在荧光显微镜下,观察FJC阳性细胞的形态和在新大脑皮质中的整体分布情况。结果在匹罗卡品处理组,许多新大脑皮质出现呈亮黄绿色荧光的FJC阳性细胞,而对照组未见。结论在小鼠匹罗卡品癫痫模型中,运用FJC染色技术在新大脑皮质中显示发生了大量神经元变性,有利于更好地理解颞叶癫痫的长期病理变化和自发反复发作的癫痫机制。Objective To detect degenerative neurons that may occur in the neocortex of pilocarpine-treated mice by Fluoro-Jade C（FJC）.Methods Ten male adult mice（Kunming strain） were divided into pilocarpine-treated group （n=5） and control goup（n=5）.The control mice were injected intraperitoneally with atropine and saline.The pilocarpine-treated mice were injected intraperitoneally with atropine and pilocarpine to induce status epilepticus（SE）.Pilocarpine-treated mice were sacrificed at 12 h after SE.The control mice were also sacrificed at the same time.Brain coronal sections in the neocortex levels were made and stained by FJC staining.Finally,FJC-stained brain sections were observed under an epifluorescence microscope to identify the shape and distribution of the FJC positive cells in the neocortex.Results In pilocarpine-treated group,the FJC-positive staining cells showed a bright green color in the somas and fine processes with neuronal profiles.Numerous degenerative cells could be seen in many neocortex regions.Above changes were not observed in the control animals.Conclusion FJC staining is successfully applied to demonstrate massive neurodegeneration in the neocortex,which will be helpful to understand of long-term brain pathological changes and recurrent seizure mechanism in temporal lobe epilepsy. [

关 键 词：FLUORO-JADE C 癫痫 匹罗卡品 神经变性 

分 类 号：R742.1[医药卫生—神经病学与精神病学]