液相色谱-串联质谱法分析正山小种红茶中茶黄素  

Analysis of Theaflavins in Black Tea of Lapsang Souchong by HPLC-MS/MS

作　　者：侯冬岩[1] 刁全平[1] 吴寒[1] 李铁纯[1] 回瑞华[1] 刘俊会[2] 

机构地区：[1]鞍山师范学院化学与生命科学学院,辽宁鞍山114007 [2]国家农业深加工产品质量监督检验中心,吉林长春130002

出　　处：《鞍山师范学院学报》2012年第4期34-37,共4页Journal of Anshan Normal University

基　　金：辽宁省教育厅科学技术基金资助课题(L2010001)

摘　　要：建立一种用于分析正山小种红茶中茶黄素的液相色谱-串联质谱法,利用多重反应监测模式对正山小种红茶中茶黄素进行定性和定量分析.色谱柱为反相C18柱,流动相为0.1%甲酸和乙腈,柱温35℃.分析结果表明:方法检出限为6.6 ng/g,相对标准偏差小于4.08%,回收率为91.2%～102.5%,该方法简便、快速、准确,适用于红茶中茶黄素的分析.A liquid chromatography coupled with tandem mass spectrometry method for the analysis of theaflavins in black tea is established.The theaflavins in black tea are identified by the HPLC-ESI-MS-MS technique with the multiple-reaction monitoring mode.The separation of theaflavins is performed on sunfire-C18 at 35 ℃,formic acid-0.1% and acetonitrile are used as the mobile phase for elution.The limit of detection is 6.6ng/g,the relative standard deviation is less than 4.08%,the recoveries are 91.2%~102.5%.The results show that the method is simple,rapid and accurate for the determination of theaflavins in black tea.

关 键 词：红茶 茶黄素 液相色谱-串联质谱 

分 类 号：O658[理学—分析化学]