Human umbilical cord blood-derived mesenchymal stem cells promote regeneration of crush-injured rat sciatic nerves  

作　　者：Mi-Ae Sung Hun Jong Jung Jung-Woo Lee Jin-Yong Lee Kang-Mi Pang Sang Bae Yoo Mohammad S. Alrashdan Soung-Min Kim Jeong Won Jahng Jong-Ho Lee 

机构地区：[1]Department of Craniofacial Structure & Functional Biology, School of Dentistry, Seoul National University, Seoul, Republic of Korea [2]Department of Occupation and Environment, Postgraduate College of Medicine, Konkuk University, Choong-ju, Republic of Korea [3]Department of Oral & Maxillofacial Surgery, School of Dentistry, Seoul National University, Seoul, Republic of Korea [4]Department of Dentistry, Oral and Maxillofacial Surgery, Ajou University School of Medicine, Suwon, Republic of Korea [5]Dental Research Institute, Seoul National University, Seoul, Republic of Korea

出　　处：《Neural Regeneration Research》2012年第26期2018-2027,共10页中国神经再生研究（英文版）

基　　金：supported by a grant of the Seoul National University Dental Hospital,Republic of Korea,No.03-2010-0020

摘　　要：Several studies have demonstrated that human umbilical cord blood-derived mesenchymal stem cells can promote neural regeneration following brain injury. However, the therapeutic effects of human umbilical cord blood-derived mesenchymal stem cells in guiding peripheral nerve regeneration remain poorly understood. This study was designed to investigate the effects of human umbilical cord blood-derived mesenchymal stem cells on neural regeneration using a rat sciatic nerve crush injury model. Human umbilical cord blood-derived mesenchymal stem cells （1 ~ 106） or a PBS control were injected into the crush-injured segment of the sciatic nerve. Four weeks after cell injection, brain-derived neurotrophic factor and tyrosine kinase receptor B mRNA expression at the lesion site was increased in comparison to control. Furthermore, sciatic function index, Fluoro Gold-labeled neuron counts and axon density were also significantly increased when compared with control. Our results indicate that human umbilical cord blood-derived mesenchvmal stem cells promote the functinnal r~.RcJv^rv nf P.n I^h-inillr^4 ~r^i~tit, n^r~eSeveral studies have demonstrated that human umbilical cord blood-derived mesenchymal stem cells can promote neural regeneration following brain injury. However, the therapeutic effects of human umbilical cord blood-derived mesenchymal stem cells in guiding peripheral nerve regeneration remain poorly understood. This study was designed to investigate the effects of human umbilical cord blood-derived mesenchymal stem cells on neural regeneration using a rat sciatic nerve crush injury model. Human umbilical cord blood-derived mesenchymal stem cells （1 ~ 106） or a PBS control were injected into the crush-injured segment of the sciatic nerve. Four weeks after cell injection, brain-derived neurotrophic factor and tyrosine kinase receptor B mRNA expression at the lesion site was increased in comparison to control. Furthermore, sciatic function index, Fluoro Gold-labeled neuron counts and axon density were also significantly increased when compared with control. Our results indicate that human umbilical cord blood-derived mesenchvmal stem cells promote the functinnal r~.RcJv^rv nf P.n I^h-inillr^4 ~r^i~tit, n^r~e

关 键 词：human umbilical cord blood-derived mesenchymal stem cells sciatic nerve crush injury FLUOROGOLD stem cells peripheral nerve regeneration REGENERATION neural regeneration 

分 类 号：R745[医药卫生—神经病学与精神病学]