广西地区原发性肝细胞癌患者甲胎蛋白基因多态性检测及其临床意义分析  

Analysis of polymorphisms of α-fetoprotein gene and its clinical implications in hepatocellular carcinoma patients from Guangxi,China

作　　者：莫国辉[1] 黄健旅[1] 梁毓[1] 黎殿德[1] 于龙[1] 刘征涛[1] 齐鲁楠[1] 覃晓[1] 彭民浩[1] 肖开银[1] 李佳荃[2] 吴华[2] 彭涛[1] 

机构地区：[1]广西医科大学第一附属医院肝胆血管外科 [2]广西医科大学科学实验中心,广西南宁530021

出　　处：《川北医学院学报》2012年第5期418-421,共4页Journal of North Sichuan Medical College

基　　金：国家自然科学基金(NSFC81072321;NSFC30960021-C010803);2009年教育部新世纪优秀人才支持计划;广西省卫生厅重点科研课题(重201018)

摘　　要：目的:检测甲胎蛋白(a-fetoprotein,AFP)基因RS12506899、RS16849384、RS7688274,三位点基因型在广西地区原发性肝细胞癌患者中的分布,探讨候选基因型对患者血清AFP水平以及临床病理指标的影响。方法:选取广西医科大学一附院和广西医科大学肿瘤医院2005年3月至2011年3月间行肝癌切除手术治疗肝细胞癌(hepatocellular carcinoma,HCC)患者177例。提取组织DNA,Taq-man法进行基因分型。对各组基因型间AFP水平及临床病理特点进行比较。结果:不同基因型各组间AFP水平、肿瘤直径、乙肝表面抗原的分布情况,差异无统计学意义。无肝硬化的HCC患者AFP水平高于合并肝硬化的HCC患者(P=0.047)。单因素生存分析结果提示P53抗体阳性程度越高,其无瘤生存时间越短(P=0.010)。结论:在本组人群中,AFP候选基因型与HCC患者的AFP水平及其临床参数不相关;P53阳性者预后较差。Objective：To genotype three loci （rs12506899 ,rs7688274,rs16849384） at α-fetoprotein （AFP） gene in Guangxi hepa- tocellular carcinoma （HCC） and to explore its association with serum AFP levels and clinical-pathological factors. Methods： 177 cases of HCC were recruited from the First Affiliated Hospital of Guangxi Medical University and the Tumor Hospital of Guangxi Medical Uni- versity from Mar 2005 to Mar 2011. All patients received liver cancer resection. Extracted DNA was subjected to genotyping by real time PCR （Taq-man） assay. Association between genotypes and serum AFP level and clinical - pathological factors were analyzed. Results： There was no statistically significant association between AFP genotypes and AFP levels. AFP levels in HCC patients without cirrhosis were higher than those with cirrhosis （ P = 0. 047）. Patients with higher scoring of TP53 staining in tumor tissues had worse survival than those with smaller scoring （P = 0. 010）. Conclusion： In this population, there was no significant association between AFP geno- types and serum AFP level and clinical - pathological factors. Patients with p53 positive staining had unfavorable outcome after tumor resection.

关 键 词：原发性肝细胞癌 甲胎蛋白 单核苷酸多态性 定量性状连锁分析 

分 类 号：R735.7[医药卫生—肿瘤]