虾青素对辛硫磷所致大鼠皮肤成纤维细胞氧化损伤的拮抗作用  

Antagonistic effects of astaxanthin on oxidative damage induced by phoxim skin fibroblasts in rats

作　　者：闫建国[1] 周亚莉[2] 王松涛[1] 方方[1] 

机构地区：[1]新乡医学院人体解剖学教研室,河南新乡453003 [2]新乡医学院医学微生物学教研室

出　　处：《环境与健康杂志》2012年第10期901-904,共4页Journal of Environment and Health

基　　金：新乡医学院高学历人才科研启动项目

摘　　要：目的探讨虾青素对辛硫磷所致大鼠皮肤成纤维细胞氧化损伤及凋亡的拮抗作用。方法取4只3日龄清洁级SD大鼠背部皮肤培养。取第4代成纤维细胞,正常对照组和辛硫磷氧化损伤组更换新鲜培养基,低、中、高剂量虾青素保护组分别更换含0.2、2、20μg/L虾青素的培养基,于37℃、5%CO2培养24 h后,正常对照组更换新鲜培养基,辛硫磷氧化损伤组和各剂量虾青素保护组分别更换含10μg/L辛硫磷的培养基,于37℃、5%CO2培养24 h。采用噻唑兰(MTT)比色法检测细胞存活率;采用流式细胞术检测细胞凋亡率;测定细胞超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活力和丙二醛(MDA)含量。结果与对照组比较,辛硫磷染毒组和虾青素保护组大鼠皮肤成纤维细胞存活率和SOD、CAT活力下降,凋亡率和MDA含量升高。与辛硫磷染毒组比较,虾青素保护组大鼠皮肤成纤维细胞存活率和SOD、CAT活力较高,凋亡率和MDA含量下降;且随着虾青素剂量的升高,辛硫磷染毒大鼠皮肤成纤维细胞存活率和SOD、CAT活力呈上升趋势,凋亡率和MDA含量呈下降趋势。结论虾青素对辛硫磷所致的大鼠皮肤成纤维细胞氧化损伤及凋亡具有明显的拮抗作用。Objective To investigate the antagonistic effects of astaxanthin on oxidative damage and apoptosis induced by phoxim skin fibroblast cells in cultured in rats. Methods The skin fibroblast cells of passage 4 of rat were incubated with astaxanthin with different concentrations （0, 0.2, ：2 and 20 μg/L） for 24 h, and then were exposed to 10 μg/L phoxim for 24 h. The cell viability was evaluated by MTT assay, and the cell apoptosis was detected by flow cytometry. The levels of SOD, CAT and MDA in cell were detected by spectrophotometry. Results Compared with the control group, the cell viabilities, SOD activity and CAT activity of the phoxim exposure group with different concentrations（0.2, 2 and 20 μg/L） of astaxanthin were decreased, the contents of MDA and the apoptosis rates of the rat skin fibroblast cells were increased. Compared with the phoxim exposure group,the cell viability,SOD activity and CAT activity of the different concentrations （0.2, 2 and 20 μg/L） of astaxanthin increased, the contents of MDA and the apoptosis rates of the rat skin fibroblast cells were decreased. With the increasing doses of astaxanthin, for the rat skin fibroblast cells of P4 exposed to phoxim, the cell viability and SOD, CAT activities had increasing trend, the rate of apoptosis and MDA content had decreasing trend. Conclusion Astaxanthin has obvious antagonistic effects on the oxidative damage and apoptosis induced by phoxim in rat skin fibroblasts in cultured.

关 键 词：虾青素 辛硫磷 皮肤成纤维细胞 氧化损伤 细胞凋亡 

分 类 号：R994.6[医药卫生—毒理学]