奇球菌属类胡萝卜素提取物的抗氧化活性研究  

SCREENING OF ANTIOXIDANT ACTIVITIES OF CAROTENOID EXTRACTS FROM Deinococcus

作　　者：王飞[1] 封琼[1] 刘程智[1] 林琳[1] 田兵[1] 华跃进[1] 

机构地区：[1]浙江大学原子核农业科学研究所/农业部核农学重点开放实验室,浙江杭州310029

出　　处：《核农学报》2012年第6期900-905,共6页Journal of Nuclear Agricultural Sciences

基　　金：国家"863"项目(2007AA021305);国家自然科学基金重点资助项目(30830006);重大新药创制科技重大专项(2009ZXJ09001-034);转基因生物新品种培育重大专项项目(2009ZX08009-075B);农业部行业专项"核技术农业应用"(200803034);中央高校基本科研业务费专项基金

摘　　要：从不同种奇球菌属(Deinococci)细菌中分离提取类胡萝卜素,并利用高效液相色谱分析了提取物的组成和性质。通过DPPH自由基和超氧自由基清除试验,比较了不同菌种来源的类胡萝卜素提取物的抗氧化能力,结果发现,D.radiopugnans和D.radiodurans提取物的体外抗氧化能力最强,在浓度为0.6μg/ml时DPPH自由基清除率都达到46%左右;D.radiopugnans提取物的超氧自由基清除活性较强,在浓度为3μg/ml时清除率达到42.99%。同时,采取体外蛋白质氧化抑制试验和脂类氧化抑制能力测定分析比较了不同种类提取物对大分子的保护作用,结果表明D.radiopugnans和D.radiodurans提取物具有较强的生物大分子氧化防护功能。以上研究为从具有极端环境适应能力的奇球菌属筛选抗氧化活性类胡萝卜素,并进一步研究其作用机制提供了基础。Carotenoids from the species of Deinococci were extracted and analyzed by using HPLC for their composition and property.By measurements of DPPH radical and superoxide radical scavenging activities,antioxidant capacities of different pigments from Deinococcus were investigated.The results showed that the carotenoid extracts of D.radiopugnans and D.radiodurans had stronger antioxidant capacities in vitro,which about 46% DPPH radicals were scavenged at the concentration of 0.6μg/ml.Carotenoid extract from D.radiopugnans showed the strongest superoxide radical scavenging activity among all the tested samples,with 42.99% of superoxide radicals were scavenged at the concentration of 3μg/ml.Furthermore,the protective effects of different extracts on biomacromolecule by inhibition of protein oxidation and protection of lipid oxidation were compared.Results showed that extracts from D.radiopugnans and D.radiodurans had strong protective abilities of biological macromolecules against oxidative damage.The present study helps screening the carotenoids with strong antioxidant capability from the extreme bacteria Deinococcus,and provides the basis for further research on their action mechanisms.

关 键 词：奇球菌属 类胡萝卜素 抗氧化活性 

分 类 号：TS201.2[轻工技术与工程—食品科学]