放线菌H50发酵液抑菌活性及其稳定性测定  

Antimicrobial Activity and Stability of Fermentation Liquid from Actinomycete H50 Strain

作　　者：黄剑[1] 李天华[1] 崔艺久[1] 白洪志[1] 韩梅[1] 

机构地区：[1]沈阳农业大学土地与环境学院,沈阳110161

出　　处：《沈阳农业大学学报》2012年第3期311-315,共5页Journal of Shenyang Agricultural University

摘　　要：将沈阳农业大学微生物研究室分离的1株放线菌H50[链霉菌属(Streptomyces)]分别以植物病原菌真菌12株、细菌3株为供试菌,采用菌丝生长速率法和管碟法测定H50发酵液的抑菌活性以及发酵液的稳定性。发酵液的抑菌活性测定结果表明:H50发酵液对12株供试病原真菌均具有不同程度抑制作用,对其中6种病原真菌菌丝生长抑制率达50%以上,对油菜菌核病菌和黄瓜灰霉病菌的抑制率分别为98.3%和94.9%;H50发酵液对3种供试病原细菌中的枯草芽孢杆菌的抑制作用最强,抑菌圈直径为12.3mm。发酵液的稳定性检测结果表明:发酵液在80℃处理后抑菌活性开始下降,发酵液在pH值4.0～8.0条件下抑菌活性稳定,可见该菌株的代谢产物具有广谱抑菌活性和较强的热、酸、紫外光稳定性,具有潜在的开发价值。The strain of H50 was isolated from the soil in the lab.By identification,the strain was regarded as streptomyces,taking 12 epiphyte,3 bacteria as object,by means of mycelium growth rate and cup-plate method,the antimicrobial activity of bioassay was studied.The stability of the fermentation broths in different conditions was measured.The antimicrobial tests showed that the inhibition rate of all the 12 strains were considerable and 6 of them reached 50%.It showed strongest inhibition effect against Bacillus subtills（12.3 mm）.Organize method tests showed that inhibition rate of fermentation broths of the strain H50 to Sclerotinia sclerotiorum and Botrytis cinerea was 98.3% and 94.9%,respectively.Through the research of stability of the fermentation broths of actinomycete strain H50 to heat,acid,alkali and light we found that it had a stable activity to heat,acid and UV,but unstable to alkali.Antimicrobial spectrum of actinomycete strain H50 was wide.Antimicrobial activity of the fermentation broths was stable and worth to be developed.

关 键 词：放线菌 发酵液 抑菌活性 稳定性 

分 类 号：S432.4[农业科学—植物病理学] S476.1[农业科学—农业昆虫与害虫防治]