ADAMTS-2及TGF-β1在肝硬化组织中的表达  

Expression of ADAMTS-2 and TGF-β1 in cirrhotic liver

作　　者：李汉军[1] 董超[2] 曹庭加[1] 常实[2] 

机构地区：[1]广州军区武汉总医院普通外科,武汉430070 [2]中南大学湘雅医院普通外科,长沙410008

出　　处：《中南大学学报（医学版）》2012年第10期1026-1030,共5页Journal of Central South University ：Medical Science

基　　金：湖南省科学技术厅项目(2011FJ6047)~~

摘　　要：目的:检测含Ⅰ型凝血酶敏感蛋白模体的解整合素样金属蛋白酶(a disintegrin and metalloprotease with thrombospondin motif,ADAMTS)-2及转化生长因子(transforming growth factor,TGF)-β1在肝硬化组织中的表达,探讨ADAMTS-2与TGF-β1在肝硬化发生和发展中的作用。方法:收集武汉总医院2010年3月至6月肝硬化门静脉高压症患者的肝组织16例及外伤性肝破裂正常肝组织8例作为正常对照组。采用免疫组织化学及Western印迹法检测ADAMTS-2及TGF-β1在肝硬化组织及正常组织中的表达。结果:免疫组织化学结果显示肝硬化组织中ADAMTS-2和TGF-β1表达较正常肝组织明显增高(P<0.05)。Western印迹分析亦显示ADAMTS-2及TGF-β1在肝硬化组织中表达强度明显高于正常组织(P<0.05),而且两者的表达呈正相关(r=0.862,P<0.01)。结论:ADAMTS-2和TGF-β1可能存在协同致肝纤维化作用。Objective： To explore the expression and distribution of a disintegrin and metalloprotease with thrombospondin motif （ADAMTS）-2 and transforming growth factor （TGF） -β1 in patients with or without cirrhosis, and to determine their relation. Methods： The liver tissues from 16 patients with cirrhotic portal hypertensive and 8 patients with liver injury were collected in Wuhan General Hospital from March to June, 2010. Immunohistochemistry and Western blot were applied to detect the protein expression of ADAMTS-2 and TGF-β1. Results： Immunohistochemistry showed that the expression of ADAMTS-2 and TGF-β1 was significantly higher in the cirrhotic tissues than that in normal tissues （P〈0.0S）. Western blot also showed the expression of ADAMTS-2 and TGF-β1 in the cirrhosis tissues was significantly higher than that in normal tissues （P〈0.0S）. there was a positive correlation between ADAMTS-2 and TGF-β1（r=0.862, P〈0.01）. Conclusion： ADAMTS-2 and TGF-β1 may have a synergistic reaction in promoting liver cin＇hosis.

关 键 词：ADAMTS TGF-Β1 肝硬化 WESTERN印迹 免疫组织化学 

分 类 号：R575.2[医药卫生—消化系统]