高效液相色谱法测定纸制食品接触材料中邻苯二甲酸酯的迁移量  

Determination of Phthalic Acid Esters Released from Food Paper Packaging Materials by High Performance Liquid Chromatography

作　　者：杨博锋[1] 汤志旭[2] 高昕[1] 牛增元[2] 罗忻[2] 王风美[2] 简慧敏[2] 

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266003 [2]山东出入境检验检疫局,山东青岛266002

出　　处：《分析测试学报》2012年第10期1272-1276,共5页Journal of Instrumental Analysis

基　　金：国家质检总局科技计划项目(2011IK101);国家自然科学基金项目(31071631)

摘　　要：以蒸馏水、3%乙酸、10%乙醇、95%乙醇4种食品替代物模拟纸制食品接触材料中10种邻苯二甲酸酯的迁移。以乙腈和水为流动相,Eclipse XDB-C18(4.6 mm×150 mm,5μm,Agilent)色谱柱分离4种食品模拟物中的10种邻苯二甲酸酯;以C18固相萃取小柱对其进行富集净化,建立了高效液相色谱/二极管阵列检测器测定这10种邻苯二甲酸酯类化合物的方法。结果表明,该10种化合物均在224 nm处有最大吸收波长,其标准曲线的线性相关系数(r2)均大于0.999 9,加标回收率为71%～107%,相对标准偏差为0.86%～8.0%,检出限均不大于0.1 mg/kg。该方法灵敏、准确,满足相关法规的限量要求。The different types of food simulated migration of ten phthalic acid esters in food paper packaging materials were replaced by distilled water, 3% acetic acid, 10% ethanol and 95% ethanol. The ten phthalic acid esters in four food simulants were enriched and purified by C18 solid phase extraction column, and separated on an Eclipse XDB -C18（4. 6 mm x 150 mm, 5 μm, Agilent）column using acetonitrile and water as mobile phases. The analysis of ten phthalic acid esters was performed by high performance liquid chromatography with a diode array detector. Under the optimal conditions, all of the ten phthalate acid esters had the same maximum absorbance at 224 nm, The calibration curves were linear in the certain concentration range with correlation coefficients （r2） higher than 0.999 9. The recoveries at spiked levels of 0.05, 0. 1, 0.2 mg . L-1 ranged from 71% to 107% with RSDs of 0. 86% - 8. 0%. The limits of detection were lower than 0. 1 mg/kg. The method was simple, rapid and reliable, and could meet the requirements for relevant limit regulations.

关 键 词：高效液相色谱 食品模拟物 纸制食品接触材料 邻苯二甲酸酯类 固相萃取 

分 类 号：O657.72[理学—分析化学] TQ414.1[理学—化学]