不同来源乳酸菌的药敏试验  

The Drug Sensitive Tests of Lactic Acid Bacteria from Different Sources

作　　者：曾小群[1] 潘道东[1,2] 郭宇星[2] 曹锦轩[1] 

机构地区：[1]宁波大学生命科学与生物工程学院,浙江宁波315211 [2]南京师范大学国家乳品加工技术研发分中心,南京210097

出　　处：《中国食品学报》2012年第9期179-185,共7页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(30972130;31101314);浙江省自然科学基金重大项目(Z3110211);浙江省自然科学基金项目(LQ12C20003);浙江省教育厅科研计划项目(Y201121436);宁波市农业创新创业资金重点项目(2010C92024);宁波市重大攻关项目(2011C11017)

摘　　要：从不同来源的材料中分离并鉴定12株乳酸菌菌株,并进行10种常用抗生素药敏检测。结果表明:耐药率较高药物为阿米卡星(100%)、氟哌酸(83.33%),敏感率最高是氯霉素(100%),中介率最高为氟哌酸(16.67%)。不同来源的乳杆菌药敏性各不相同,其中新疆来源的菌株对阿米卡星和氟哌酸的耐药率为100%。不同菌种的药敏性也有差异:干酪乳杆菌的耐药性较广,为33.33%～91.67%。所有菌种均对氟哌酸和阿米卡星有耐药性,对氯霉素敏感。在不同来源和菌种这两种因素中,来源是影响菌株药敏性的更主要因素。乳酸乳球菌乳亚种LQ-12、瑞士乳杆菌15019、戊糖乳杆菌SC-1、布氏乳杆菌SC-2、干酪乳杆菌S1、发酵乳杆菌A1、发酵乳杆菌B3、发酵乳杆菌B5含有质粒,这几株乳杆菌可能含有可转移抗药性基因。本试验结果为所分离到的乳酸菌应用于生产实践及合理地使用抗生素提供了指导意义。12 strains of lactic acid bacteria （LAB） were screened from different sources and were taking drug sensitive tests of 10 common antibiotics. The results indicated that： the antibiotics of highest resistance ratio were amikacin （100%）, norfloxacin （83.33%）; the antibiotic of highest sensitive ratio was chloramphenicol （100%）; the antibiotic of highest intermediat ratio was norfloxacin （16.67%）. The LAB from different sources were with different drug sensitivities. The LAB from Xinjiang Province had resistance ratio of 100% to amikacin and norfloxacin. Different LAB had different drug sensitivities. Lactobacillus casei had broader drug resistance ratio of 33.33%-91.67%. All species of LAB were resistant to amikacin and norfloxacin and sensitive to chloramphenicol. The source was the more important factor than species to influence the drug sensitivity of LAB. Lactococcus lactis lactis LQ-12, Lactobacillus helveticus 15019, Lactobacillus pentosus SC-1, Lactobacillus butcheri SC-2, Lactobacillus casei $1, Lactobacillus fermentum A1, Lactobacillus fermentum B3, LactobaciUus fermentum B5 had plasmids and they might have transferable genes of antibiotics resistance. The results of these tests provided direction significance of using the isolated LAB in practice and Using antibiotics reasonably.

关 键 词：乳酸菌 药敏试验 耐药 敏感 质粒 

分 类 号：R446.5[医药卫生—诊断学]