卡拉胶的荧光光谱特性及其质量浓度预测  

Study on the Fluorescence Spectra of Carrageenan and Prediction of Its Concentration

作　　者：黄奇峰[1] 高淑梅[1] 陈国庆[1] 陈超[1] 孔凡标[1] 李润[1] 

机构地区：[1]江南大学理学院,江苏无锡214122

出　　处：《江南大学学报（自然科学版）》2012年第5期592-596,共5页Joural of Jiangnan University (Natural Science Edition)　

基　　金：国家自然科学基金项目(61178032);江苏省科技支撑计划(社会发展)项目(BE2011828);中央高校基本科研业务专项基金项目(JUSRP31005);高等学校博士学科点专项科研基金项目(200802950005);江南大学研究生科研创新计划项目

摘　　要：针对卡拉胶在食品加工中作为增稠剂、乳化剂和稳定剂使用,而大量摄入会对身体造成严重损害,文中对卡拉胶的三维荧光光谱进行了系统研究。结果表明,其在390 nm处存在一个激发峰,分别在485 nm、620 nm、660 nm处存在荧光峰,其中660 nm峰较强、485 nm峰较宽且红移,提出瑞利散射和水的拉曼散射的影响导致485 nm谱峰的红移。进而发现卡拉胶溶液荧光光谱强度随质量浓度的增加而增强,并通过主成分分析-BP神经网络训练,实现了对卡拉胶质量浓度的预测,准确率达到90%以上。当质量浓度高于120μg/mL时,准确率达到98%以上,表明该技术可快速测定溶液中卡拉胶的含量。Carrageenan is used for thickener, stabilizer, and texturizer in the industry of food processing, but over-intake will seriously do harm to our health. The three-dimensional fluorescence spectra of carrageenan solution are obtained by using FLS920 fluorescences spectrometer. The fluorescence emission peaks of earrageenan are located at 485 nm, 620 nm and 660 nm and the excitation peak is at 390 nm. The red shift of the 485 nm peak with the excitation wavelength increases may be caused by the Rayleigh scattering and the Raman scattering of water. The fluorescence intensity of different concen- tration of earrageenan solution is attained. The principal component analysis and the BP neural network are used to predict the concentration of different carrageenan solution. Results show that the accuracy is greater than 90% and even greater than 98% when the concentrations are higher than 120 ixg/mL. This method can provide a quick method for the measure- ment of the content of carrageenan.

关 键 词：荧光光谱 主成分分析 BP神经网络 卡拉胶 

分 类 号：O657.3[理学—分析化学]