前列腺癌组织雄激素受体N端区基因突变的研究  

The study of N-terminal dormain of mutation of androgen receptor gene in prostate cancer tissues

作　　者：于小玲[1] 卢建[2] 王晓慧 陈光椿 赵子文[1] 

机构地区：[1]山东省滨州医学院病理生理学教研室,滨州市256603 [2]第二军医大学基础部病理生理学教研室,上海市200433

出　　处：《肿瘤防治杂志》2000年第2期143-145,共3页China Journal of Cancer Prevention and Treatment

基　　金：国家自然科学基金!(396 70 30 0 )资助

摘　　要：目的 :研究雄激素受体 (AR)基因突变与前列腺癌 (PC)发生、发展的关系。方法 :采用自行设计的 3对引物 ,检测了 2 5例PC石蜡切片组织 ,对其AR的N端转录激活区进行了银染聚合酶链式反应 单链构象多态性分析 (PCR SSCP)和异常SSCP片段的DNA测序。结果 :发现一患者的A片段有SSCP异常 ,经测序证实A外显子存在一错义突变 (C96 6 →A ,Ser2 96Arg)。结论 :这是一新发现的突变 ,这一突变位于转录激活区内 ,推测可改变AR的转导活性 ,从而影响AR的功能。Objective:To study the relationship between the mutation of androgen receptor(AR) gene and the development of human prostate cancer(hPC).Methods:With the 3 pairs of primers designed by ourselves,we detected the transcriptive dormain of N terminal of AR gene in 25 paraffin embedded slides of hPC by polymerase chain reaction single strand conformation polymerphism(PCR SSCP).The abnormal shift band was further detected by direct DNA cycle sequencing.Results:One mobility difference was found by SSCP.From this shift band we identified one point mutation in 296 amino acid (C 966 →A,Ser296Arg).Conclusions:It's an unreported point mutation.This result might change the transcriptive activity of AR.Further studies are needed to evaluate the effects of this mutation on AR function.

关 键 词：前列腺癌 雄激素受体 基因突变 

分 类 号：R737.25[医药卫生—肿瘤]