Development and validation of RP-HPLC method for estimation of eplerenone in spiked human plasma  

作　　者：Paraag Gide Sandeep Sonawane Abhishek Chitnis 

机构地区：[1]MET's Institute of Pharmacy,MET League of Colleges,Bhujbal Knowledge City,Nashik 422003,Maharashtra,India [2]Department of Clinical Sciences and Administration,University of Houston,College of Pharmacy,Houston,TX 77030,USA

出　　处：《Journal of Pharmaceutical Analysis》2012年第5期390-393,共4页药物分析学报（英文版）

摘　　要：A rapid and simple high performance liquid chromatography （HPLC） mcthod wiih a UV detection （241 nm） was developed and validated for estimation of eplerenone from spiked human plasma. The analyte and the internal standard （valdecoxib） were extracted with a mixture of dichloromethane and diethyl ether. The chromatographic separation was performed on a HiQSil C-18HS column （250 mm × 4.6 mm, 5 um） with a mobile phase consisting of acetonitrile：water （50：50, v/v） at flow rate of 1 mL/min. The calibration curve was linear in the range 100 3200 ng/mL and the heteroscedasticity was minimized by using weighted least squares regression with weighting factor I/X.A rapid and simple high performance liquid chromatography （HPLC） mcthod wiih a UV detection （241 nm） was developed and validated for estimation of eplerenone from spiked human plasma. The analyte and the internal standard （valdecoxib） were extracted with a mixture of dichloromethane and diethyl ether. The chromatographic separation was performed on a HiQSil C-18HS column （250 mm × 4.6 mm, 5 um） with a mobile phase consisting of acetonitrile：water （50：50, v/v） at flow rate of 1 mL/min. The calibration curve was linear in the range 100 3200 ng/mL and the heteroscedasticity was minimized by using weighted least squares regression with weighting factor I/X.

关 键 词：EPLERENONE Liquid-liquidextraction Weighted regression HPLC-UV 

分 类 号：R927[医药卫生—药学]