番茄红素对铁负荷大鼠抗氧化功能影响  

Protective effects of lycopene on oxidative stress in iron overload rats

作　　者：王瑞[1] 张红[2] 吴博[1] 冯彦红[1] 刘重斌[1] 

机构地区：[1]温州医学院基础医学院生理教研室,浙江温州325035 [2]湖州师范学院医学院

出　　处：《中国公共卫生》2012年第11期1457-1459,共3页Chinese Journal of Public Health

基　　金：浙江省自然科学基金(Y2110388);浙江省科技厅公益性应用研究项目(Y2011C37091);温州市科技项目(Y20100330)

摘　　要：目的探讨番茄红素对铁负荷大鼠抗氧化功能保护作用。方法 36只SD大鼠按体重随机分为空白对照、铁负荷、番茄红素、番茄红素高、中、低剂量干预6组,每组6只,持续饲喂6周后,取血和肝、肾、脾、心和结肠组织测定铁含量,总铁结合力;测定血清和组织中丙二醛(MDA)、总超氧化物歧化酶(T-SOD)、过氧化氢酶(CAT)及谷胱甘肽-S转移酶(GST)活性。结果高剂量番茄红素干预组大鼠血清、肝、脾、心脏和结肠组织中铁含量分别为(19.06±2.1)μmol/L、(25.67±2.7)μg/g、(38.30±4.8)μg/g、(17.30±2.6)μg/g和(12.08±2.9)μg/g,与铁负荷组比较,高剂量番茄红素干预组大鼠组织中铁含量明显下降,血清总铁结合力明显增加,MDA含量明显下降,T-SOD活性明显上升,血清、心脏、肝和结肠CAT、GST活性明显上升(P<0.05)。结论番茄红素能有效清除体内自由基,增强机体抗氧化能力。Objective To examine protective effects of lycopene on oxidative stress.Methods Thirty-six SD rats were randomly divided into control,iron,lycopene,iron＋high lycopene,iron＋medium lycopene,and iron＋low lycopene group（6 rats in each group） and fed for 6 weeks.The iron concentration and total iron-binding capacity（TIBC） in serum and liver,kidney,spleen,heart,and colon tissue of the rats were determined.At the same time,the levels of malondialdehyde（MDA）,total superoxide dismutase（T-SOD）,catalase（CAT）,and glutathione S-transferase（GST） were measured.Results In iron＋high lycopene group,iorn concentrations of serum and liver,spleen,heart,and colon tissue were 19.06±2.1 μmol/L,25.67±2.7,38.30±4.8,and 12.08±2.9 μg/g.In the high lycopene group,the serum iron decreased significantly;the serum TIBC increased significantly;the content of MDA decreased significantly;the activity of T-SOD increased significantly;and the activities of CAT and GST in serum,heart,liver and colon increased significantly compared to those of the iron load group（P0.05 for all）.Conclusion The results suggest that lycopene prevents iron-induced oxidative stress with its potent free radical scavenging and antioxidant properties.

关 键 词：番茄红素 铁负荷 氧化应激 大鼠 

分 类 号：R151.1[医药卫生—营养与食品卫生学]