生物法制备2,3-丁二醇的最新进展  

Latest Advances of Microbial Production of 2,3-Butanediol

作　　者：付晶[1] 王萌[1] 刘维喜[1] 陈涛[1] 

机构地区：[1]天津大学化工学院系统生物工程教育部重点实验室,天津300072

出　　处：《化学进展》2012年第11期2268-2276,共9页Progress in Chemistry

基　　金：天津市自然科学基金项目(No.12JCYBJC12900);国家自然科学基金项目(No.21176182);教育部博士点基金项目(No.20100032120014);国家重点基础研究发展计划(973)项目(No.2011CBA00804;2012CB725203)资助

摘　　要：2,3-丁二醇及其衍生物作为重要的液体燃料和化工原料,具有广阔的工业应用前景。高效、经济的2,3-丁二醇生物制备方法,对我国低碳经济和循环经济的建设具有重要的促进作用。针对近三年间生物法制备2,3-丁二醇领域的最新研究成果,本文综述了当前国内外学者在该领域研究的热点,即关键基因和酶的鉴定、新菌种的开发和代谢工程改造、同步糖化和共培养等发酵条件的优化、耦合工艺等分离纯化技术改进等。使用非致病的高产单一2,3-丁二醇手性异构体的代谢工程菌株,作为细胞炼制工厂,利用廉价的非粮原料作底物,采用经济、简单、环保的分离纯化方式,是2,3-丁二醇产业化发展的可靠保障。As important liquid fuel and chemical raw materials, 2,3-butanediol and its derivatives have broad industrial application prospects. Economical and efficient 2,3-butanediol microbial production has significant impetus to the low-carbon and circular economy development of China. Focusing on the latest achievements in the microbial production of 2, 3-butanediol, this review summarizes the hot spots of the researchers＇ attentions nowadays, which can be divided into four parts ： identification of the key genes and enzymes involved in the 2,3- butanediol metabolic pathway, development of new strains and metabolic engineered strains for high yield or chiral 2,3-butanediol production, strategies for fermentation optimization such as simultaneous saccharification and fermentation, and technology improvement for combining process involved in 2,3-butanediol recovering processing. These achievements in the last three years are classified and discussed with state-of-art views. At last, guidelines for future studies are also proposed. It is pointed out that future research should focus on new strains, new genes and enzymes, new metabolic pathways, new cheap and renewable resources for substrate, and new technology for fermentation and separation. There＇s no doubt that metabolic engineered class I strains, which can utilize cost- effective and renewable substrates to lower the raw materials cost producing chiral 2,3-butanediol with high yield and productivity, should be favored. Besides, new technology in separation and purification process must be developed and improved to lower the cost of downstream processing.

关 键 词：2 3-丁二醇 手性异构体 代谢工程 合成生物学 可再生资源 发酵 

分 类 号：Q5[生物学—生物化学] TQ923[轻工技术与工程—发酵工程]