富含miR-30a的牛初乳组分通过抑制癌细胞自噬促进顺铂化疗的肿瘤消亡  

miR-30a Enriched Colostrum-Fraction Can Inhibit The DDP-inducedAutophagy of HeLa Cells and Promote The Tumor Shrink

作　　者：邹珍友[1] 汪洋[1] 丁寒莹[1] 吴临平[1] 陈淑美[1] 蒋挺[1] 国芳[1] 张鹤云[1] 陈熹[1] 张骑鹏[1] 张辰宇[1] 曾科[1] 

机构地区：[1]江苏小分子核糖核酸工程研究中心,南京大学生命科学学院医药生物技术国家重点实验室,南京210093

出　　处：《生物化学与生物物理进展》2012年第11期1099-1108,共10页Progress In Biochemistry and Biophysics

基　　金：国家重点基础研究发展计划(973)(2012CB517603,2011CB504803863);国家自然科学基金(90813035)资助项目~~

摘　　要：本实验首次发现牛初乳中富含miR-30a.取牛初乳中富含miR-30a的组分处理用顺铂化疗的癌细胞,能显著地抑制癌细胞自噬,促进癌细胞凋亡:单独使用顺铂化疗18 h的癌细胞凋亡率为12.5%,而化疗的同时施加富含miR-30a的牛初乳组分18 h后癌细胞凋亡率为56.5%.用这种富含miR-30a的牛初乳组分饲喂顺铂化疗的荷瘤小鼠,能更有效地抑制肿瘤生长:化疗小鼠饲喂牛初乳组分后,肿瘤直径自6 mm缩小为4.5 mm,而单独用顺铂化疗的肿瘤直径约8 mm.因此富含miR-30a的牛初乳组分能增进化疗药物的疗效.施用牛初乳组分,还可减少化疗药物的用量:单独用顺铂处理细胞18 h需20 mg/L剂量,加牛初乳组分后10 mg/L即可取得明显凋亡效果;单独用顺铂化疗荷瘤小鼠10天每次需500μg,而添加富含miR-30a的牛初乳组分后,顺铂剂量减半即可明显缩小肿瘤.Here we firstly report that colostrum contains a high level of miR-30a.Using the miR-30a rich fraction extracted from colostrum,we successfully restrained the autophagy activity of HeLa cells induced by DDP,and promoted their apoptosis from 12.5% to 56.5% in 8 h post-treatment of DDP.By feeding the tumor-bearing mice undergoing DDP-chemotherapy with miR-30a-rich fraction from colostrum for 10 days,we reduced tumors size more effective than treating mice with DDP alone.In particular,the diameter of tumors was diminished to 4.5 mm after miR-30a-rich colostrum fraction administration during DDP chemotherapy,while the tumors treated with DDP only grown to 9.0 mm in diameter.In conclusion,the miR-30a-rich fraction of colostrum can sensitize the cancer cells to anti-cancer drugs,thus enhance the effect of chemotherapy drug on tumor treatment.

关 键 词：miR-30a 自噬 细胞凋亡 牛初乳 顺铂 

分 类 号：Q279[生物学—细胞生物学] R730.54[医药卫生—肿瘤]