紫茎泽兰挥发油对金黄色葡萄球菌的抗菌机制  

Studies on antibacterial mechanism of the volatile oils from Eupatorium adenophorum Spreng on Staphylococcus aureus

作　　者：刘松鑫[1] 韦会平[2] 程君[2] 杨俊卿[1] 

机构地区：[1]重庆医科大学,重庆400016 [2]攀枝花学院,四川攀枝花617000

出　　处：《中国医院药学杂志》2012年第21期1742-1745,共4页Chinese Journal of Hospital Pharmacy

摘　　要：目的:研究紫茎泽兰挥发油对金黄色葡萄球菌的抗菌机制。方法:通过测定培养液的电导率和大分子物质的OD值、SDS/PAGE电泳、DAPI染色后的荧光观测,研究紫茎泽兰挥发油对金黄色葡萄球菌细胞形态、可溶性蛋白的表达和核酸合成的影响。结果:紫茎泽兰挥发油作用前后,金黄色葡萄球菌培养液的电导率和大分子物质的OD值增加,SDS/PAGE电泳谱带和DAPI染色后的荧光现象均产生显著变化。结论:紫茎泽兰挥发油抗菌机制可能涉及影响金黄色葡萄球菌细胞膜完整性、可溶性蛋白表达和核酸合成。OBJECTIVE To study antibacterial mechanism of the volatile oils from Eupatorium adenophorum Spreng（EAVO）on Staphylococcus aureus.METHODS Electrical conductivity detection,SDS/PAGE electrophoresis and DAPI staining assay were used to primarily explore the antibacterial mechanism of EAEO against S.aureus.RESULTS After treated with EAVO,electrical conductivity,SDS/PAGE and DAPI staining of S.aureus obviously changed.CONCLUSION The antibacterial mechanism of EAEO may involve in affecting cell membrane integrity,soluble protein and nucleic acid synthesis of S.aureus.

关 键 词：紫茎泽兰 挥发油 抗菌机制 

分 类 号：R378.11[医药卫生—病原生物学]