动物源性食品鸭血、猪血DNA提取方法研究及双重PCR检测  

Study on DNA Extraction of Animal Origin Food Duck Blood and Pig Blood and Detection by Double PCR

作　　者：周正 吕二盼[2,3] 周巍 张薇 邢文静[3] 柳毅[3] 

机构地区：[1]河北省食品质量监督检验研究院,石家庄050091 [2]河北农业大学食品科技学院,保定071001 [3]保定市产品质量监督检验所,保定071051

出　　处：《食品工业》2012年第11期161-166,共6页The Food Industry

基　　金：河北省质量技术监督局重点项目(100102)

摘　　要：研究从鸭血、猪血中提取DNA的快速简便方法并建立双重PCR鉴别方法。用改进的氯仿-醋酸钠(NaAc)提取法和KI提取法从固体块状鸭血中提取DNA,与经典酚仿抽提法进行对比,经PCR扩增检测提取效果。建立双重PCR方法鉴别动物源性食品中的鸭血、猪血成分,并对市售动物源性血制品进行检测。这两种改进的DNA提取方法得到的DNA纯度较高,凝胶电泳条带整齐,背景清晰;PCR反应能扩增出目的条带。双重PCR能同时扩增出鸭和猪的条带。这两种改进的DNA抽提方法能获得高纯度DNA,比传统方法安全、简便、节省试剂,PCR扩增结果很好,应用双重PCR方法能同时检测出血样制品中的鸭、猪成分。Study onduck-blood and pig blood-a-imed to-find a-quickand eas; way-io extraci-DNA-andestablish PCR method for identification. The improved Chloroform-NaAc method and KI extraction method used for DNA extracting were compared with the classic Phenol extraction method by PCR amplification from the solid massive duck blood. A double PCR method was established to identify the duck, pig blood components in animal origin food and carried on the examination to animal blood products from the market. The gel electrophoresis stripes indicated that the two improved DNA extraction methods were of high purity; the target bands were clear and neat and can be amplified by PCR. Double PCR simultaneously amplified duck and pig bands. The two improved DNA extraction methods can obtain the DNA of high purity, they are safe, convenient and economical compared with the traditional method. PCR amplification result is good, the application of duplex PCR method can also detect duck and pig components in the blood product.

关 键 词：鸭血 猪血DNA 核酸提取 双重PCR检测 

分 类 号：TS251.93[轻工技术与工程—农产品加工及贮藏工程]