苗药走马胎超临界CO_2流体萃取物中挥发性成分的GC-MS分析  

Chemical Constituents of the Volatile Oil from the Root of Cinnamomum camphora by Supercritical Carbon Dioxide Fluid Extraction by Gas Chromatography-Mass Spectrometry

作　　者：杨碧仙[1] 胡奇志[2] 胡馨 云雪林[2] 

机构地区：[1]贵阳学院生物与环境工程系,贵阳550005 [2]贵阳中医学院药学院,贵阳550002 [3]贵州高原彝药厂,贵阳550004

出　　处：《中国实验方剂学杂志》2012年第22期127-131,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：贵阳学院2008年度科研项目(2008036)

摘　　要：目的:研究苗药走马胎的挥发性化学成分。方法:采用超临界CO2萃取法(SFE)从苗药走马胎中萃取挥发性成分,萃取条件为:萃取釜压力25 MPa,温度50℃,萃取时间2 h。用气相色谱法-质谱法(GC-MS)分离测定萃取物,结合计算机检索鉴定化合物结构,应用色谱峰面积归一化法计算各成分相对百分含量。结果:SFE萃取物得率1.7%,鉴定出54个化学成分,匹配度>90%的成分38个,占挥发油总数的70.37%,主要为黄樟脑(29.44%)、榄香素(23.58%)、谷甾醇(12.43%)、亚麻油酸(11.32%)、安息香酸苄酯(4.64%)、洋橄榄油酸(3.37%)等。结论:苗药走马胎超临界CO2流体萃取条件及其挥发性成分的GC-MS研究,为利用和深入研究该药提供了依据。Objective： To study the chemical constituents of the volatile oil from the root of Cinnamomum camphora. Method： The essential oil was obtained by supercritical carbon dioxide fluid extraction （SFE）. Extraction conditions were： extracting 2 h at 50 ℃ , 25 MPa. Separation and determination of the extract were performed by GC-MS, structures were identified with computer retrieval technology, calculated the relative content of the extract with chromatographic peak area normalization method. Result： The extraction efficiency of SFE was 1.7% , 54 chemical constituents were separated and identified, 39 chemical compositions which matching degree above 90% , accounting for 70.37% of total volatile oil. Main constituents include safrole （29.44%） , elemicin （23.58%） , stigmast-5-en-3-ol （ 12.43% ） , linoleic acid （ 11.32% ） , benzyl benzoate （4.64%） , elaidinic acid （3.37%）, etc. Conclusion： This paper reports the conditions of supercritical carbon dioxide fluid extraction of the root C. camphora and research the composition of volatile oils by GC-MS for the first time. Provides a basis for the use and in-depth study.

关 键 词：苗药走马胎 挥发性成分 超临界CO2萃取 气相色谱-质谱联用 

分 类 号：R284.1[医药卫生—中药学]