响应面分析法优化1-脱氧野尻霉素液相色谱检测的衍生化条件  

Optimization of derivatization conditions for the determination of 1-deoxynojirimycin by response surface methodology

作　　者：王腾[1] 李樾[2] 李翠清[1] 张亚运[1] 李继文[1] 郭晓燕[1] 

机构地区：[1]北京石油化工学院制药工程系,北京102617 [2]中国食品药品检定研究院,北京100050

出　　处：《食品工业科技》2012年第22期63-67,共5页Science and Technology of Food Industry

基　　金：教育部科学技术研究重点项目(211003);北京市教育委员会科技发展计划面上项目(KM201110017002)

摘　　要：在1-脱氧野尻霉素的高效液相色谱法分析中,利用响应面分析法优化了荧光衍生化的最佳条件。根据Box-Behnken中心组合实验设计原理,在单因素实验基础上,以1-脱氧野尻霉素回收率为响应值,应用响应面分析法研究衍生化反应时间、反应温度和衍生化试剂用量各因素间的交互作用及对1-脱氧野尻霉素回收率的影响。确定最佳衍生化条件为:衍生化反应时间22min、衍生化反应温度29℃、衍生化试剂用量0.43mmol/L,实际回收率为99.4%。For the purpose of establishing a high performance liquid chromatography method for the determination of 1-deoxynojirimycin（1-DNJ）,the optimal derivatization conditions were studied by response surface methodology. Based on the result of single factor experiments,the Box-Behnken design was used to investigate the effect of derivative time,temperature and the amount of derivative reagent on the recovery of 1-deoxynojirimycin. The derivative conditions were found to be 22min,29℃ and 0.43mmol/L and the recovery was 99.4%.

关 键 词：1-脱氧野尻霉素 衍生化 响应面分析法 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]