邻苯二甲醛荧光衍生化法测定食用海藻中还原型谷胱甘肽  

Determination of glutathione in edible marine algae using OPA fluorescence derivatizing method

作　　者：李铉军[1] 韩玲[1] 朱爱花[1] 崔胜云[1] 

机构地区：[1]延边大学长白山生物资源与功能分子教育部重点实验室,吉林延吉133002

出　　处：《食品工业科技》2012年第22期81-83,91,共4页Science and Technology of Food Industry

基　　金：国家自然科学基金(21165021)

摘　　要：用质谱和光谱测定方法研究了OPA和还原型谷胱甘肽(GSH)荧光衍生化反应,发现OPA分子苯环上相邻醛基的歧化反应,使得其与GSH发生衍生化反应时生成三环和二环结构的两种衍生化产物,其中三环结构的衍生化产物具有较强的荧光发射特性。利用OPA荧光衍生化法分别测定了马尾藻、鹿角菜、龙须菜中GSH的含量。在含OPA的PBS缓冲溶液、激发波长λex=350nm时,样品溶液在428nm处发射灵敏的荧光,检测限达3.6×10-8mol/L。用标准加入法分别测定样品中GSH含量分别为:0.0714mg/g(马尾藻),0.1183mg/g(鹿角菜),0.1970mg/g(龙须菜),方法的回收率达到99.22%～100.41%。The derivatizing reaction between OPA and reduced glutathione（GSH） were investigated using ESI-MS,spectrometric analysis.Because of Cannizaro reaction of OPA in the solution,two derivitized products had been formed with conjugated tricyclic structure and bicyclic structure,in which much more fluorescent emmision properties was observed by derivatized product with tricyclic conjugated structure.In PBS buffer soluiton,using OPA as derivatizing agent,GSH content in Sargassum siliquastrum,Silvetia siliquosa,Gracilaria lemaneiformis had been determined by fluorometryic analysis.In OPA＋PBS mixture buffer solution with excitation wavelength at 350nm,the sample emmited strong fluorescence at 428nm,the detection limit was 3.6×10-8mol/L.GSH contents determined with standard addition methods in the samples were 0.0714mg/g（Sargassum siliquastrum）,0.1183mg/g（Silvetia siliquosa）,0.1970mg/g（Gracilaria lemaneiformis） respectively,and the reconveries were in the range of 99.22%～100.41%.

关 键 词：谷胱甘肽 邻苯二甲醛 马尾藻 鹿角菜 龙须菜 荧光发射 

分 类 号：O652.3[理学—分析化学]