果糖月桂酸单酯的分离纯化及鉴定  

Purification and identification of monolauroyl fructose

作　　者：付敏[1] 赵强忠[1] 仇超颖[1] 刘宁[1] 赵谋明[1] 

机构地区：[1]华南理工大学轻工与食品学院,广东广州510640

出　　处：《食品工业科技》2012年第22期276-279,283,共5页Science and Technology of Food Industry

基　　金：粤港招标项目(2009A020700003);国家863计划项目(2010AA101505)

摘　　要：采用硅胶柱层析色谱对叔戊醇中固定化磷脂酶Lecitase Ultra催化合成的果糖月桂酸单酯进行分离纯化。确定了薄层层析(TLC)条件,并通过反相-高效液相色谱-蒸发光散射检测(RP-HPLC-ELSD)测定柱层析纯化产物的纯度。利用液质联用(HPLC-ESI-MS)、红外光谱(FT-IR)和13C核磁共振(13CNMR)对目标产物进行确认,鉴定目标产物为酯化位点在1位和6位的果糖月桂酸单酯,四种异构体如下:1-月桂酸-β-D-吡喃果糖单酯、1-月桂酸-α-D-吡喃果糖单酯、1-月桂酸-β-D-呋喃果糖单酯和6-月桂酸-β-D-呋喃果糖单酯,且1-月桂酸-β-D-吡喃果糖单酯的比例最高。A novel enzymatic-catalyzing process of monolauroyl fructose was developed.In the process immobilized Lecitase Ultra was used as biocatalyst in anhydrous 2-methyl-2-butanol.Silica gel column chromatography was used to separate and purify the fructose ester.The qualitative analysis condition of TLC was confirmed and the purity was identified by RP-HPLC-ELSD.Structure was verified by HPLC-ESI-MS,FT-IR,13C NMR.As a result,a mixture of the C-1 and C-6 monoacylated frutose esters,four isomers of monolauroyl fructose were obtained：l-lauroyl-β-D-fructopyranose,1-lauroyl-α-D-fructopyranose,1-lauroyl-β-D-fructofuranose,6-lauroyl-β-D-fructofuranose,and l-lauroyl-β-D-fructopyranose was the highest proportion.

关 键 词：果糖月桂酸单酯 固定化磷脂酶Lecitase ULTRA 薄层层析 硅胶柱层析 结构 

分 类 号：TS202.3[轻工技术与工程—食品科学]