结直肠癌组织SPARC和MMP-2及VEGF的表达临床意义分析  

Relationship and clinical significance of SPARC,MMP-2 and VEGF protein expression in colorectal cancer tissues

作　　者：戴芳芳[1] 于国华[2] 张居民[3] 王文辉[2] 齐元玲[2] 殷晓燕[2] 

机构地区：[1]潍坊医学院研究生学院,山东潍坊261041 [2]潍坊市人民医院肿瘤内科,山东潍坊261041 [3]潍坊市人民医院病理科,山东潍坊261041

出　　处：《中华肿瘤防治杂志》2012年第14期1086-1090,共5页Chinese Journal of Cancer Prevention and Treatment

摘　　要：目的:检测结直肠癌(CRC)组织中富含半胱氨酸的分泌性酸性蛋白质(SPARC)、基质金属蛋白酶-2(MMP-2)和血管内皮生长因子(VEGF)的表达状况,探讨其与CRC临床病理因素、预后的关系。方法:采用通用型二步法测定60例CRC中SPARC、MMP-2和VEGF的表达情况。结果:SPARC在CRC肿瘤细胞的表达低于肿瘤间质的表达,χ2=14.545,P=0.000。SPARC在肿瘤细胞低表达者,无进展生存期(PFS)和总生存期(OS)都短于高表达者,P=0.000。相反,MMP-2高表达者,PFS(P=0.024)和OS(P=0.022)都短于低表达者。VEGF高表达者,PFS短于低表达者,P=0.029,而OS差异无统计学意义,P=0.139。结论:SPARC在肿瘤细胞的低表达促进了CRC的侵袭转移,其可能促进了MMP-2和VEGF的表达,因此检测SPARC、MMP-2和VEGF的表达情况可预测CRC的预后。OBJECTIVE： To explore the expressions of SPARC MMP-2 and VEGF in colorectal cancer tissues,and analyze the relationship and the clinical significance. METHODS： Universal twostep method was used to examine the expressions of SPARC, MMP-2 and VEGF in 60 cases colorectal cancer specimens. RESULTS： The expression of SPARC in tumor cells was lower than that in stromal cells （χ2 = 14. 545, P=0. 000）. Survival analysis results showed that the level of SPARC＇s expression was related to patients’ prognosis. Both progression free survival （PFS） and over survival （OS） in lower expression group were shorter than those in higher expression group （P=0. 000）. Adversely, patients had shorter PFS（P=0. 024）and OS（P=0. 022）in higher expression MMP-2, and shorter PFS in higher expression of VEGF （P=0. 029） while the OS had no significant （P=0. 139）. CONCLUSIONS： Low level expression of SPARC may promote the invasion and metastasis of colorectal cancer and the mechanism is related to increased expression of MMP-2 and VEGF,which leads to degradation of matrix, damage of basement membrane and formation of new blood vessels. To explore the expression of SPARC, MMP-2 and VEGF may forecast patients’ prognosis.

关 键 词：结直肠肿瘤 病理学 SPARC MMP-2 VEGF 预后 

分 类 号：R735.3[医药卫生—肿瘤]