番茄红素诱导人结肠癌SW480细胞凋亡的作用机制  

Mechanism of Lycopene Induced Cell Apoptosis on Human Colon Cancer SW480 Cells

作　　者：赵其辉[1] 邱青朝[2] 胡波[1] 

机构地区：[1]湖南环境生物职业技术学院护理学院,湖南衡阳421005 [2]南华大学医学院

出　　处：《肿瘤防治研究》2012年第11期1306-1310,共5页Cancer Research on Prevention and Treatment

基　　金：湖南环境生物职业技术学院院长科研基金资助项目(Z08-02)

摘　　要：目的探讨番茄红素诱导人结肠癌SW480细胞凋亡的分子机制。方法体外培养SW480细胞,采用MTT比色实验、流式细胞术、RT-PCR和Western blot方法,分析番茄红素对SW480细胞的生长增殖作用、对细胞凋亡率的影响及对细胞凋亡相关分子Bcl-2、Bax、Caspase-9和Caspase-3表达的影响。结果 MTT法显示,番茄红素能显著抑制SW480细胞的生长增殖能力,且呈一定的量效与时效关系,24 h的半数抑制浓度(IC50)为1.0μmol/L;流式细胞仪分析表明,番茄红素呈浓度依赖性诱导SW480细胞凋亡;RT-PCR或Western blot分析表明,SW480细胞经不同浓度番茄红素诱导后,与溶媒组相比,Bcl-2表达逐渐减弱,而Bax,Caspase-9和Caspase-3表达逐渐增强,Bcl-2/Bax比值降低。结论番茄红素对SW480细胞的抑制增殖作用,可能与降低Bcl-2/Bax比值、增加Caspase-9和Caspase-3蛋白表达、诱导细胞凋亡有关。Objective To investigate the effect of Lycopene on the apoptosis of human colon cancer SW480 cells and the relevant molecular mechanism.Methods The anti-proliferative effect of a different concentration of Lycopene on SW480 cells was measured by MTT assay.Ratio of SW480 cells apoptosis was analyzed by flow cytometry.The levels of Bcl-2,Bax were detected by RT-PCR and Western blot.The expression levels of Caspase-9 and Caspase-3 were analyzed by Western blot.Results The MTT assay showed that Lycopene had potent anti-proliferative effect on SW480 cells in a time-and dose-dependent manner.The IC50 value of Lycopene was SW480 cells treated with 1.0 μmol/L Lycopene for 24 hours.Flow cytometry analysis revealed that Lycopene increased the percentage of apoptotic SW480 cells.The expression level of Bcl-2 were decreased,while the expression levels of Bax,Caspase-9 and Caspase-3 were increased significantly in SW480 cells treated with different concentrations of Lycopene.Conclusion Lycopene could induce apoptosis of SW480 cells by decreasing the ratio of Bcl-2/Bax and increasing Caspase-9 and Caspase-3 expression.

关 键 词：番茄红素 结肠癌 SW480细胞 细胞凋亡 

分 类 号：R735.3[医药卫生—肿瘤] R965.1[医药卫生—临床医学]