‘红阳’猕猴桃MYB基因的克隆与表达  

Cloning and expression analysis of MYB in Actinidia chinesis 'Hongyang'

作　　者：满玉萍[1] 李刚[1] 刘虹[1] 王彦昌[2] 覃瑞[1] 

机构地区：[1]中南民族大学生命科学学院,武汉430074 [2]中国科学院植物种质创新与特色农业重点实验室,武汉430074

出　　处：《华中农业大学学报》2012年第6期679-685,共7页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金项目(3067143;31171945)

摘　　要：为探讨MYB基因在‘红阳’(Actinidia chinesis‘Hongyang’)猕猴桃果实着色过程中的重要作用,利用反转录聚合酶链式反应(RT-PCR)结合快速扩增cDNA末端(RACE)技术克隆了‘红阳’猕猴桃的一个MYB转录因子基因。该基因的cDNA全长962bp,序列包含一个666bp的开放阅读框(ORF),编码221个氨基酸残基的蛋白质,其N端具有2个典型的MYBDNA结合域。同源性分析显示,该酶的氨基酸序列与矮牵牛、葡萄、番茄、金鱼草等植物中花青素途径相关的MYB转录因子基因的相似性都达到80%以上。实时荧光定量PCR分析结果显示,AcMYB在‘红阳’猕猴桃中的表达量与花青素含量呈正相关,二者均在果实转色主要阶段维持较高水平,推测该基因在调控花青素合成的过程中起着重要作用。To elucidate the function of MYB in Actinidia chinesis ＇Hongyang＇ pigmentation,a novel MYB gene（AcMYB） was isolated from that cultivar by reverse transcription-polymerase chain reaction（RT-PCR） and rapid amplification of cDNA ends（RACE）.The full-length cDNA of AcMYB is 962 bp with an open reading frame（ORF） of 666 nucleotides encoding a protein of 221 amino acids with two typical MYB DNA binding domains at its N-terminal.The result of Blast X showed that AcMYB had high similarity with MYB transcription factors in Petunia hybrida Vilm.,Vitis spp.,Solanum lycopersicum L.,Antirrhinum majus L.and sequences from other plant species related to the elevation of anthocyanin pigmentation.Real-time PCR analysis indicated that the expression of MYB was positively correlated with the main anthocyanin concentration in Actinidia chinesis ＇Hongyang＇ and both of them maintained high levels at the stage of pigmentation,suggesting that AcMYB may play an important role in regulating anthocyanin biosynthesis.

关 键 词：花青素 猕猴桃 MYB 基因克隆 表达分析 

分 类 号：S663.4[农业科学—果树学]