Localization of Vibrio vulnificus infection in dendritic cells and its effects on the cytoskeleton  

作　　者：WANG Zhi-gang XU Shui-ling SHAO Ping-yang BAO Yi CUI Ge CAI Yu-jie 

机构地区：[1]Department of Clinical Laboratory, Second Hospital of Jiaxing, Jiaxing, Zhejiang 314000, China [2]Jiaxing Medicine College, Jiaxing, Zhejiang 314000, China

出　　处：《Chinese Medical Journal》2012年第23期4264-4269,共6页中华医学杂志（英文版）

摘　　要：Background Vibrio vulnificus （Vv） is an estuadne bacterium that can cause primary septicemia as well as serious wound infections. However, little is known about the mechanisms by which Vv infects dendritic cells （DCs） and its effects on cytoskeleton. In this study, we aimed to investigate the invasion, internalization, and the organelles damage of the cultured dendritic cells （a DC 2.4 strain） during Vv infection. Methods The study model was the cultured DCs infected by a Vv 1.758 strain. Electron microscopy was used to observe the localization of bacteria at the different time points of infection, cell morphology, and the process of organelles changes. The cytoskeleton structure including the microfilaments and the microtubules rearrangement was examined under a fluorescence microscope. Results The Vv were pinocytosised into the DC cells through double-sides, and localized at 1-2 μm of the inner side membrane. It took 1.3, 1.9, and 3.4 hours to reach the infection ratio of 25%, 50%, and 75%, respectively. Using electron microscopy, the DCs had been observed to have developed chromatin aggregation within 4.0 hours, and significant cytoskeleton structure disruption was noted within 6.0 hours.Background Vibrio vulnificus （Vv） is an estuadne bacterium that can cause primary septicemia as well as serious wound infections. However, little is known about the mechanisms by which Vv infects dendritic cells （DCs） and its effects on cytoskeleton. In this study, we aimed to investigate the invasion, internalization, and the organelles damage of the cultured dendritic cells （a DC 2.4 strain） during Vv infection. Methods The study model was the cultured DCs infected by a Vv 1.758 strain. Electron microscopy was used to observe the localization of bacteria at the different time points of infection, cell morphology, and the process of organelles changes. The cytoskeleton structure including the microfilaments and the microtubules rearrangement was examined under a fluorescence microscope. Results The Vv were pinocytosised into the DC cells through double-sides, and localized at 1-2 μm of the inner side membrane. It took 1.3, 1.9, and 3.4 hours to reach the infection ratio of 25%, 50%, and 75%, respectively. Using electron microscopy, the DCs had been observed to have developed chromatin aggregation within 4.0 hours, and significant cytoskeleton structure disruption was noted within 6.0 hours.

关 键 词：vibrio vulnificus dendritic cells CYTOSKELETON MICROFILAMENT MICROTUBULE 

分 类 号：R515.3[医药卫生—内科学]