一株好氧反硝化细菌的分离鉴定及反硝化特性研究  

Isolation and Identification of an Aerobic Denitrifier and Its Denitrifying Characteristic

作　　者：郭端强[1] 刘海龙[1] 万亚涛[1] 李小卫[1] 陈艳艳[1] 管丽冰[1] 单林娜[1] 

机构地区：[1]河南城建学院,平顶山467000

出　　处：《生物技术通报》2012年第10期205-209,共5页Biotechnology Bulletin

基　　金：河南省科技攻关重点项目(092102310069)

摘　　要：通过对采集水样进行富集培养,利用溴百里酚蓝(BTB)选择性培养基初筛和活性测定复筛得到一株好氧反硝化细菌N22',发现该菌在好氧条件下能有效去除培养液中的NO3--N。硝酸盐氮初始浓度为125 mg/L,培养40 h后硝酸盐氮去除率达86.39%;扫描电镜照片显示,菌株N22'为短杆菌,无鞭毛,大小约为(0.75-1.25)μm×(0.5-0.75)μm范围内,菌落表面呈乳白色。通过形态、生理生化特征及16S rRNA基因序列分析,初步判断菌株N22'为不动杆菌属Acinetobactersp.。反硝化性能测试结果表明,该菌反硝化作用的最适温度为25-30℃,pH值7.0。An aerobic denitrifying bacterial strain N22＇ was isolated from water using selective medium.The reduction efficiency was up to 86.39% under aerobic conditions after 40 hours respectively with initial NO3——N concentration of 125 mg/L.The morphology of strain N22＇ was a brevibacillus with an average size of（0.75-1.25） μm×（0.5-0.75） μm and an ivory-white surface.N22＇ was identified as Acinetobacter sp.by morphological/biochemical characteristics and molecular properties.The denitrifying experiment results showed that the optimal conditions for the aerobic denitrification were about 25-30℃ and at pH7.0.

关 键 词：好氧反硝化 不动杆菌 16S RRNA 系统发育 

分 类 号：X703[环境科学与工程—环境工程]