高脂膳食诱导的大鼠肥胖对小肠黏膜糖吸收功能的影响  

Impact of high-fat diet induced obesity on glucose absorption in small intestinal mucose in rats

作　　者：黄微[1] 刘瑞[1] 郭韡[1] 魏娜[1] 强鸥[1] 李献[1] 欧艳[1] 唐承薇[1] 

机构地区：[1]四川大学华西医院生物治疗国家重点实验室,人类疾病相关多肽研究室,华西医院消化内科,成都610041

出　　处：《卫生研究》2012年第6期878-882,共5页Journal of Hygiene Research

基　　金：国家自然科学基金(No.30870919);四川省科技厅科技支撑计划(No.2010SZ0176)

摘　　要：目的探讨高脂膳食诱导肥胖的发生是否与小肠黏膜糖类消化及吸收功能的改变相关联。方法 46只雄性SD大鼠随机分为高脂组(n=31)与正常对照组(n=15),分别用高脂饲料和基础饲料饲养24周。24周后高脂饲料组大鼠根据体重分为肥胖组(n=16)及肥胖抵抗组(n=10)。测定大鼠的体重及腹腔脂肪湿重、空腹血糖水平、小肠黏膜麦芽糖酶及蔗糖酶活性。免疫组织化学法、RT-PCR法及蛋白质免疫印迹法检测大鼠小肠黏膜中Na+-依赖型葡萄糖转运蛋白(SGLT-1)的表达水平。结果肥胖组大鼠的体重、腹腔脂肪湿重、空腹血糖水平、小肠黏膜麦芽糖酶活性及SGLT-1蛋白表达量显著高于正常对照组及肥胖抵抗组(P<0.05)。3组大鼠小肠黏膜蔗糖酶活性无明显差异(P>0.05)。肥胖组大鼠小肠黏膜SGLT-1 mRNA的表达水平与正常对照组及肥胖抵抗组比较分别增加了12.5%和23%,但差异无显著性(P>0.05)。结论高脂膳食诱导的大鼠肥胖与小肠黏膜中麦芽糖酶活性增强及糖吸收的关键分子SGLT-1的表达增加相关联。Objective To investigate whether high-fat diet induced obesity was associated with variation of glucose absorption in small intestinal mucosa of rats.Methods 46 male SD rats were randomly divided into high-fat diet group（n=31） and control group（n=15）,fed with high-fat diet and normal diet for 24 weeks,respectively.After 24 weeks,the rats were divided into obese（n=16） and obesity-resistant group（n=10） according to their body weight.Rats＇body weight,abdominal fat weight,plasma glucose level,maltase,sucrase activity in small intestinal mucosa were measured.SGLT-1 expression in intestinal mucosa was detected by immunohistochemistry,RT-PCR and Western blot.Results Mean body weight,abdominal fat weight,fast plasma glucose levels,maltase activities and SGLT-1 protein expression in intestinal mucosa of obese rats were significantly higher than those in the control and obesity-resistant rats（P0.05）.Sucrase activities in intestinal mucosa showed no statistical difference among the three groups（P0.05）.The SGLT-1 mRNA expression in obese group was increased by 12.5% and 23% when compare with the control and obesity-resistant group,respectively.But the difference was not statistical significant（P0.05）.Conclusion High-fat diet induced obesity was associated with the increased intestinal maltase activity and expression of SGLT-1 in rats,the key molecule in glucose absorption.

关 键 词：肥胖 小肠吸收 Na+-依赖型葡萄糖转运蛋白 麦芽糖酶 大鼠 

分 类 号：R151.2[医药卫生—营养与食品卫生学] Q591.4[医药卫生—公共卫生与预防医学]