酶解法辅助浸渍提取两面针中总生物碱的工艺优选  

Optimization of Extracting Technology for Total Alkaloids from Zanthoxylum nitidum by Enzymatic Hydrolysis Assisted Immersion Method

作　　者：陆世惠[1] 邓凤云[2] 卢红梅[1] 陈楠[1] 林瑶[1] 

机构地区：[1]右江民族医学院药学系,广西百色533000 [2]广西百色市中医医院,广西百色533000

出　　处：《中国实验方剂学杂志》2012年第23期43-45,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：右江民族医学院课题(右医科字[2011]1号)

摘　　要：目的:优选酶解法辅助浸渍提取两面针中总生物碱的工艺。方法:以总生物碱提取率为指标,采用单因素试验考察酶的种类、加酶量、pH、酶解温度及时间对酶解工艺的影响;以总生物碱提取率为指标,采用正交试验考察提取次数、提取时间及乙醇体积分数对两面针总生物碱浸渍提取工艺的影响。结果:酶解工艺条件为纤维素酶、果胶酶与底物质量比均为1∶250,4倍量pH 5缓冲液,室温(30℃)下酶解30 min;最佳浸渍提取工艺为以12,4,3倍量60%乙醇溶液(盐酸质量浓度5 g·L-1)室温下浸渍3次,每次2 h,总生物碱提取率85.22%。结论:该优选的工艺简单高效、节能环保,为工业生产提供实验依据。Objective： To optimize extracting process of total alkaloids from Zanthoxylum nitidum by enzymatic hydrolysis assisted immersion. Method： With extraction rate of total alkaloids as index, effect of 5 factors on enzymatic technology was investigated by single factor test, including kind of enzyme, enzyme dosage, pH, temperature and time; extraction times, extraction time ＇and the concentration of ethanol were chosen as factors, effects of 3 factors on immersion technology of total alkaloids from Z. nitidum was optimized by orthogonal test. Result： Optimized enzymatic hydrolysis process was as following： cellulase-substrate and pectinase-substrate of 1： 250, 4 times buffer with pH 5, enzyme pretreating for 30 min at ambient temperature （30 ℃ ） ; Optimum immersion extracting technology was following： extracted 3 times with 60% ethanol of 12, 4, 3 times amount （hydrochloric acid of 5 g· L^-1 ） , at ambient temperature, 2 h each time. Extraction rate of total alkaloids of 85.22% by this process. Conclusion： This optimized process was simple, efficient, energy saving and environmentally friendly, and it could provide experimental base for industrial production.

关 键 词：两面针 总生物碱 酶法 正交试验 浸渍 

分 类 号：R283.6[医药卫生—中药学]