癌性胸水p16基因纯合性缺失检测的临床意义  

The clinical significance of deletion of p16 gene homozygous deletion in malignant pleural effusion

作　　者：桂淑玉[1] 汪渊[1] 刘虎[1] 周青[2] 

机构地区：[1]安徽医科大学分子生物学实验室,安徽合肥230032 [2]安徽医科大学附属医院呼吸内科,安徽合肥230022

出　　处：《癌症》2000年第3期253-255,共3页Chinese Journal of Cancer

基　　金：安徽省教委自然科学研究基金!(96-JL077);安徽医科大学附属医院科研基金资助

摘　　要：目的 :研究癌性胸水 p16基因纯合性缺失检测的临床意义。 方法 :应用PCR技术检测胸水 p16基因第一、二外显子纯合性缺失 ,并结合胸水脱落细胞学检测分析其在临床诊断中的意义。结果 :表明所检 31例肺癌所致癌性胸水标本中均无出现 p16基因第一外显子纯合性缺失 ,12例有p16基因第二外显子纯合性缺失 ,阳性率为 38 71% (12 / 31)。 16例脱落细胞学检测阳性 ,阳性率为 5 1 6 2 % (16 / 31) ;15例脱落细胞学检测阴性 ,其中 6例存在p16基因第二外显子纯合性缺失 ;两者联合检测 ,阳性率提高至 70 97% (2 2 / 31)。统计学分析表明 p16基因第二外显子纯合性缺失和脱落细胞学检测阳性率间无明显差异 ,而两者联合阳性检出率明显高于单独p16基因第二外显子纯合性缺失 (P <0 0 1)和脱落细胞学检测 (P <0 0 1)。 2 1例结核性胸水中均无胸水 p16基因纯合性缺失。结论 :本组资料说明胸水 p16基因第二外显子纯合性缺失与脱落细胞学联合检测可增加诊断的阳性率 。Objective:To investigate the clinical significance ofhomozygous deletion of p16 gene in malignant pleural effusionMethods:The homozygous deletion of p16 gene exon1 and exon2 was examined by polymerase chain reaction (PCR) in 31 specimens of pleural effusions from lung cancer patients and compared with the determination of exfoliated cell in same specimens fromwhich the clinical value was analysed in clinical diagnosisResults:No homozygous deletion of p16 gene exon1 was found in 31 malignant pleural effusion samplesThe homozygous deletion of p16 gene exon2 was detected in 12 sampls from31 pleural effusions, while cytology positivity was found in 16 of all examined pleural effusionsThe positive rate in 31 malignant pleural effusion samplesby the determination of homozygous deletion of p16 gene and the examination of exfoliated cell was 3871%(12/31) and 5162%(16/31), respectivelyThe positive rate could be enhanced from 5162(16/31) to 7097%(22/31) by using both ofabove methods, There was no significnt difference of positive rate between homozygous deletion of p16 gene exon2 and exfoliated cytology The overall positive rate of both examination was higher than that of only the determination of homozygous deletion of p16 gene exon2 or exfoliated cytology in malignant pleural effusionNo homozygous deletion of p16 gene was found in 21 tuberculosis pleural effusion samplesConclusions:Our present data suggest that combining examinations of exfoliated cytology and homozygous deletion of p16 gene exon2 in pleural effusion can not only increase the positive rate of diagnosis, but also recruit and enhance the diagnostic value of conventional pleural cytology

关 键 词：纯合性缺失 PCR 胸腔积液 癌症 P16基因 

分 类 号：R730.4[医药卫生—肿瘤]