纳米Fe_3O_4协同海藻酸钠固定化桑叶多酚氧化酶  

Immobilization of polyphenol oxidase from mulberry leaf with sodium alginate and magnetic nanometer Fe_3O_4

作　　者：李全文[1] 严东[1] 侯杰[1] 杨洋[1] 

机构地区：[1]广西大学生命科学与技术学院,广西南宁530004

出　　处：《现代化工》2012年第10期58-61,共4页Modern Chemical Industry

基　　金：广西自然科学基金项目(2010GXNSFC013004)

摘　　要：探索磁性纳米Fe3O4协同海藻酸钠固定化桑叶多酚氧化酶。通过单因素和正交试验优化固定化条件,并采用电镜技术对固定化桑叶多酚氧化酶进行形态观察。试验结果表明最佳固定化条件为:按质量比3∶5加入磁粉纳米Fe3O4到4%海藻酸钠溶液里,在质量分数为1%氯化钙中包埋1.0 h,再用质量分数为1.5%戊二醛交联1.5 h,此条件下的酶活回收率为93.44%。通过与非磁性的海藻酸钠固定化酶的对比,磁性纳米Fe3O4复合海藻酸钠固定化酶包覆良好,酶活回收率提高了7.84%。The immobilization of polyphenol oxidase from mulberry leaf with sodium alginate and magnetic nanometer Fe3O4 is investigated in this study. The optimization of the immobilized conditions is carried out by single factor and orthogonal test. The existence state of immobilized polyphenol oxidase from mulberry leaf is analyzed by SEM. The results show that, the optimal immobilized conditions are shown as follows ：3：5 mass ratio of magnetic powder to 4% sodium alginate solution,embedding 1 h in 1% calcium chloride and erosslinking 1.5 h with 1.5% glutaraldehyde. The recovery rate of enzyme activity is 93.44%. Comparing with nonmagnetic sodium alginate immobilized enzyme, magnetic sodium alginate immobilized enzyme have good embedding effect and the activity recovery can be increased by 7.84%.

关 键 词：桑叶多酚氧化酶 磁性纳米Fe3O4 固定化 正交试验 

分 类 号：TS201.2[轻工技术与工程—食品科学]