辣椒素对肝星状细胞增殖凋亡的影响  

Effect of capsaicin on apoptosis of hepatic stellate cells

作　　者：符竣惠[1] 俞富祥[1] 汪洋[1] 徐启纲[1] 尹丽慧[1] 张启瑜[1] 

机构地区：[1]温州医学院第一附属医院肝胆外科,浙江温州325000

出　　处：《肝胆胰外科杂志》2012年第6期466-469,共4页Journal of Hepatopancreatobiliary Surgery

基　　金：浙江省外科学重中之重学科基金资助项目(2008-255)

摘　　要：目的探讨辣椒素(capsaicin)对大鼠肝星状细胞(hepatic stellate cell,HSC)T6细胞系生长的影响及其机制。方法用CCK-8(Cell Counting Kit-8)法检测终浓度50、100、150、200μmol/L的辣椒素对大鼠肝星状细胞T6细胞株生长的影响;采用流式细胞仪检测辣椒素对大鼠肝星状细胞T6细胞凋亡率的影响;Western blotting法检测Bcl-2、Bax、细胞色素酶c(Cyt c)蛋白的表达。结果辣椒素能显著抑制大鼠肝星状细胞T6细胞株增殖,呈时间浓度依赖性(P<0.05)。辣椒素作用24 h后T6细胞凋亡率与对照组相比明显升高(P<0.05),与对照组相比,辣椒素作用组Bcl-2蛋白表达量明显下降,bax蛋白的表达明显上升,Cyt c蛋白的表达明显上升,Bcl-2/Bax灰度值明显下降(P<0.05)。结论辣椒素抑制T6细胞增殖并诱导其凋亡,其机制可能是与下调Bcl-2蛋白的表达,上调Bax蛋白的表达,促进Cyt c蛋白的释放有关。Objective To probe the effect of capsaicin on hepatic stellate cells （HSC） T6 and to analyze itspossible mechanism. Methods The effect of capsaicin on T6 was measured with CCK-8 colorimetric assay; apoptosis was detected with flow cvtometrv. Bcl-2, Bax and Cvt-c were detected with Western blotting. Results Capsaicin could inhibit T6 ceils growth, which was time and concentration dependent （P〈0.05）. Annexin V assay indicated that the apoptosis rates after 24 h in different capsaicin groups were significantly higher than that of control group （P〈0.05）. Compared with control Bcl-2 was decreased obviously, Bax and Cvt c were increased obviously, BcI-2/Bax ratio was decreased obviously （P〈0.05）. Conclusion Capsaicin can inhibit the growth of T6 and induces its apoptosis, its mechanism maybe relate to down-regulation the expression of Bcl-2 and up-regulation the expression of Bax, increasing the release of Cvt c.

关 键 词：辣椒素 肝星状细胞 凋亡 B淋巴细胞瘤-2 Bcl-2相关x蛋白 细胞色素酶c 

分 类 号：R575.2[医药卫生—消化系统]