Effects of Instantaneous High-pressure on Denaturalization of β-Lactoglobulin  

瞬变高压对β-乳球蛋白变性的影响

作　　者：付敏[1] 王武[1] 方红美[1] 左君[1] 何青云[1] 陈从贵[1] 

机构地区：[1]合肥工业大学生物与食品工程学院,安徽合肥230009

出　　处：《Animal Husbandry and Feed Science》2009年第1期22-26,共5页动物与饲料科学（英文版）

基　　金：安徽省自然科学基金(070411022)~~

摘　　要：[ Objective] The study aimed to explore the denaturalization law of β-Iactoglobulin （β-LG） under instantaneous high-pressure（IHP） con- dition. [Methed] Using fresh milk as testing material, effects of pressure, feed temperature and treatment time on the denaturalization of β-LG were studied via SDS-PAGE electrophoresis and sulfhydryl content detection after IHP treatment. [ Result] IHP could induce the agglomeration and depo- lymerization Of β-LG. The agglomeration of the β-LG increased with the elevated pressure from 20 to 60 MPa, but 80 MPa pressure led to depolyme- rization. The effect of feed temperature （25 -45 ℃） was similar to that of the pressure, while the influence of treatment time （2 -8 min） was not obvious. [ Conclusion] The study provided a theoretical basis for the elimination of allergens in β-LG and the application of IHP technology in milk treatment.[ Objective] The study aimed to explore the denaturalization law of β-Iactoglobulin （β-LG） under instantaneous high-pressure（IHP） con- dition. [Methed] Using fresh milk as testing material, effects of pressure, feed temperature and treatment time on the denaturalization of β-LG were studied via SDS-PAGE electrophoresis and sulfhydryl content detection after IHP treatment. [ Result] IHP could induce the agglomeration and depo- lymerization Of β-LG. The agglomeration of the β-LG increased with the elevated pressure from 20 to 60 MPa, but 80 MPa pressure led to depolyme- rization. The effect of feed temperature （25 -45 ℃） was similar to that of the pressure, while the influence of treatment time （2 -8 min） was not obvious. [ Conclusion] The study provided a theoretical basis for the elimination of allergens in β-LG and the application of IHP technology in milk treatment.

关 键 词：β-Iactoglobulin Instantaneous high-pressure SDS-PAGE Sulfhydryl group 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]