硒对辐射诱发HPRT基因突变的保护作用  

PROTECTIVE EFFECT OF SELENIUM ON RADIATION-INDUCED HPRT MUTATION

作　　者：樊飞跃[1] 杨素霞[1] 曹珍山[1] 李煜[1] 刘国廉[1] 

机构地区：[1]北京放射医学研究所,100850

出　　处：《辐射防护》2000年第4期254-256,共3页Radiation Protection

摘　　要：本研究应用细胞质分裂阻滞法 ,检测了 60 Coγ射线对人胚肺细胞 HPRT基因突变的损伤效应 ,分析了硒对 γ射线照射所致细胞 HPRT基因突变损伤效应的保护作用。人胚肺细胞经不同剂量 60 Coγ射线照射后 ,HPRT基因突变变异子数 ,在各剂量组与对照射组相比均明显增加。细胞 HPRT基因突变变异子数随着剂量增加而逐渐增加 ,两者呈良好的线性关系。人胚肺细胞与 1× 1 0 - 7mol/L 硒作用 2 4h后接受照射 ,细胞 HPRT基因突变变异子数与单纯 60 Coγ射线照射组相比明显下降 ,说明硒对辐射所致人胚肺细胞 HPRT基因突变损伤效应具有防护效果。硒对于辐射所致的 HPRT基因突变的保护作用在接受较低剂量照射组较高剂量照射组更加明显。这种特性对于辐射防护领域所关心的小剂量照射人群的医学防护具有特殊的意义。Mutation of HPRT in human embryo lung cells induced by γ ray was analyzed by way of CB. Efficiency of HPRT mutation was gradually increased with increasing of γ ray doses after irradiation. The relationship between mutation efficiency and radiation dose showed linear relative. The efficiency of HPRT mutation in cells exposed to γ ray and selenium was lower than that in cells exposed to γ ray only. This indicated that selenium possessed protective effect on HPRT mutation induced by γ ray. This protective effect of selenium was more efficient when the cells were exposed to lower dose of γ ray than to higher dose. The character of protective effect of selenium is very significant in medical protection on people exposed to low dose of radiation (

关 键 词：电离辐射 HPRT 硒保护 基因突变 辐射防护 

分 类 号：Q6[生物学—生物物理学]