嗜酸乳杆菌及其灭活菌粘附及拮抗牙周致病菌特性研究  

The study of the adhesion and antagonistic effects of lactobacillus acidophilus with different states

作　　者：丁琴凤[1] 马丽[1] 冯希平 

机构地区：[1]上海交通大学医学院附属第九人民医院.口腔医学院口腔预防儿童科.上海市口腔医学重点实验室,200011

出　　处：《现代口腔医学杂志》2012年第6期378-382,共5页Journal of Modern Stomatology

基　　金：上海市科学技术委员会支持项目(09JC1409000);上海市重点学科建设项目资助(S30206)

摘　　要：目的研究体外共培养时,嗜酸乳杆菌(lactobacillus acidophilus,La)活菌及其灭活菌对牙龈角化上皮细胞株KB细胞的粘附,以及La对口腔致病菌具核梭杆菌(fusobacteriurn nuclearum,Fn)和牙龈卟啉单胞菌(porphyromonas gingivalis,Pg)的粘附拮抗作用,以期为牙周疾病益生菌防治提供实验依据。方法选取不同生命状态(活菌、灭活菌)的La4356作为研究菌,运用光镜以及革兰染色法,分析不同状态的嗜酸乳杆菌对口腔牙龈上皮细胞株KB细胞的粘附指数,以及La作用于Pg和Fn后,Pg和Fn粘附指数的变化。结果两种生命状态的嗜酸乳杆菌对KB细胞均有粘附作用,活菌的粘附指数略高于灭活菌。La和其灭活菌均可与Pg和Fn竞争对KB细胞的粘附,显著降低Pg和Fn对KB细胞的粘附指数;同时在允许浓度范围内,高浓度的益生菌的粘附拮抗作用较低浓度时明显。当益生菌和致病菌浓度比大于1:1时,La灭活菌抑菌作用略强于La活菌。结论益生菌La及其灭活菌均可粘附于上皮细胞,且均对Pg、Fn具有较强的粘附拮抗作用,可用作牙周生态防治。Objective To evaluate the adhesion characteristic of Lactobacillus acidophilus（La）with different states to KB cell line and their competition inhibition effects on Porphyromonas gingivalis（Pg） and Fusobacteriurn nuclearum（Fn）.Methods For the La adherence test,KB cell cultured on cover-slips incubated with La4356 were tested for 1h,3h,6h,12h,24h and 48h at 37℃.After the incubation,the cover-slips were fixed with methanol and stained with Grams stain.Fifty cells were counted for each specimen under the microscope.For the antagonistic test,the suspension of Pg,Fn mixed with La of different states were cultured with KB cell had to be cultivated together for 1h,50 invaded cells were counted for each specimen under the microscope,too.The data were analyzed by t test using SPSS 13.0 software package.Results Both live and heat-killed La could adhere to KB cell and inhibit the growth of Pg and Fn（P 0.05）,and there was slight difference between live and heat-killed.The adhesion of live La is much higher than the heat-killed.Both live and heat-killed La can significantly reduce the adhesion of Pg and Fn,and the group with La of high concentration shows a more stronger antagonism effect.The heat-killed La demonstrates a more significant antagonistic effect when its concentration is more than the pathogens（over 1：1）.Conclusion Both live and heat-killed La can adhere to KB cells and inhibit the adhesion of Pg and Fn.The new strategy of confining periodontal pathogens with La as ecological modulator is worthy of further study.

关 键 词：嗜酸乳杆菌 KB细胞 牙周致病菌 粘附 粘附拮抗 

分 类 号：R781.4[医药卫生—口腔医学]