日粮能量水平对乌金猪肝脏组织脂类代谢相关基因表达的影响  

Effect of Dietary Energy Levels on the Expression Levels of Hepatic Lipid Metabolism Genes in Wujin Pigs

作　　者：黄英[1] 李永能[2] 杨明华[1] 潘洪彬[1] 赵素梅[1] 高士争[1] 

机构地区：[1]云南农业大学,云南省动物营养与饲料重点实验室,云南昆明650201 [2]云南农业大学招生就业处,云南昆明650201

出　　处：《云南农业大学学报（自然科学版）》2012年第6期826-832,共7页Journal of Yunnan Agricultural University:Natural Science

基　　金：云南省自然科学基金重点项目(2005C0008Z)

摘　　要：为探讨日粮能量水平对乌金猪肝脏组织脂类代谢基因表达的影响。选取体重约15 kg的乌金猪54头,随机分为3组,设3个能量水平,分别为低能量水平(消化能11.84 MJ/kg)、中能量水平(消化能12.97 MJ/kg)和高能量水平(消化能14.18 MJ/kg)。30,60,100 kg体重时屠宰,取肝脏组织,采用RT-PCR方法,检测脂类代谢基因的表达水平。结果显示:高能量组乌金猪肝脏组织乙酰辅酶A羧化酶(ACC)、脂肪酸合成酶(FAS)和固醇调节元件结合蛋白(SREBP-1c)基因的表达水平显著高于中和低能量组(P<0.05),但肉碱棕榈酰转移酶(CPT-I)和过氧化物酶体增殖物激活受体-α(PPAR-α)基因的表达水平显著低于中和低能量组(P<0.05)。该研究结果将为乌金猪的科学合理饲养提供理论依据。The aim of the study was to investigate the effects of dietary energy level on expression lev- els of hepatic lipid metabolism genes in Wujin pigs. Fifty-four Wujin pigs with the body weight of 15 kg were randomly assigned into three groups and imposed on three different energy levels, which were low energy （DE： 11.84 MJ/kg）, middle energy （DE： 12. 97 MJ/kg） and high energy （DE： 14. 18 MJ/kg） respectively. At the body weights of 30, 60 and 100kg, pigs were slaughtered and liver samples were taken for analyzing the expression levels of lipid metabolism genes by RT-PCR. The re- sults showed that the expression levels of acetyl-CoA carboxylase （ ACC）, fatty acid synthase （FAS） and sterol regulatory element binding protein 1 c （SREBP-1 e） genes in high dietary energy group were significantly higher than low and middle energy groups （P 〈 0.05 ）. On the other hand, high energy could significantly reduce the expression of carnitine palmitoyltransferase I （ CPT-I）, peroxisome pro- liferator-activated receptor α（PPAR α） genes （P 〈 0. 05 ）. This study would supply the theoretical foundation for the scientific feeding of Wujin pigs.

关 键 词：能量水平 肝脏组织 脂类代谢 基因表达 乌金猪 

分 类 号：S828.912.15[农业科学—畜牧学]