肝癌1号染色体等位基因杂合性缺失及临床意义  

Significance of loss of heterozygosity chromosome 1 in hepatocellular carcinoma

作　　者：李升平[1] 张昌卿[2] 王辉云[3] 冯启胜[3] 余杏娟[3] 黄平[3] 黄丽惜[3] 冯凯涛[2] 陈敏山[1] 郭荣平[1] 林小军[1] 张亚奇[1] 李锦清[1] 李国辉[1] 曾益新[4] gzsums.edu.cn 

机构地区：[1]中山医科大学肿瘤防治中心肝胆科,广东广州510060 [2]中山医科大学肿瘤防治中心中心实验室,广东广州510060 [3]中山医科大学肿瘤防治中心肿瘤病理研究室,广东广州510060 [4]中山医科大学肿瘤防治中心肿瘤研究所

出　　处：《癌症》2000年第7期674-677,共4页Chinese Journal of Cancer

基　　金：国家"九五攻关"基金!96-907-03-02

摘　　要：目的：研究肝癌1号染色体等位基因杂合性缺失（Lossofheterozygosity，LOH）及其临床意义。方法：采用PCR及微卫星多态性技术，对65例肝癌1号染色体上28个微卫星标志位点杂合性缺失进行检测。结果：65例肝癌中63例为杂合子，57例至少在1个微卫星标志位点上发生LOH，1号染色体LOH率为91％（57／63），1p76％（48／63），1q88％（5／59）；1p36．32－p36．33和1q12～42区域内各微卫星标志LOH均在30％以上。微卫星标志LOH与性别、年龄、乙型肝炎病毒感染、肿瘤大小、包膜、血清AFP浓度、Edmonson分型均无关。D1S468和D1S2797LOH与肝癌根治术后3年内转移和复发有关，有D1S468（1p36．33）LOH者术后转移、复发率较无D1S468LOH者高（分别为82％和38％，P＜0．05）；有D1S2797（1p32）LOH者术后转移、复发率较无D1S2797LOH者高（分别为75％和37％，P＜0．05）。1p、1q及各微卫星标志位点LOH均与肝癌术后生存率无关。结论：肝癌中存在1p36．32－p36．33和1q12～42的高频LOH，其中后者为首次报道；1p36．33和1p32区域内可能存在与肝癌转移、复发有关的肿瘤抑制基因。Objective: To investigate the correlation between loss of heterozygosity (LOH) of chromosome 1 and clinicopathological parameters in hepatocellular carc inoma(HCC). Methods: LoH of chromosome 1 were detected by PCR based microsatellite polymorphism analysis technique using 28 pairs of microsatellite markers primers in 65 HCC. Results:Sixty three out of 65 cases were informative (heterozygosity), only 2 cases were no-informative. LoH of chromosome 1,1p and 1q were 91% (57/63), 76% (48/63), and 88% (52/59), respectively. More than 30% LOH were shown for microsatellite loci on regions both 1p36. 32 ~ p36. 33 and 1q12 ~ q42. LOH of microsatellite loci had no significant relation to gender, age, infection of HBV (hepatitis B virus), tumor size, capsule, concentration of AFP(α-fetoprotein), and Edmonson's classification(P > 0. 05 ). Postoperative metastasis and recurrence in cases with LOH on both D1S468 (1p36. 33 )and D1S2797(1p32) were significantly higher than cases without LOH on these two loci (82%, 75% vs 38%, 37%,respectively). There were no significant correlation between LOH of chromosome 1 and patients survival (P > 0. 05).Conclusions: There are two high frequency of LoH regions,1p36. 32 ~ p36. 33 and 1q12 ~ q42, and the later region has not been previously identified in HCC. Some tumor suppressor genes relating to tumor metastasis and recurrence may harbor in regions including 1p36. 33 and 1p32.

关 键 词：肝肿瘤 1号染色体 染合性缺失 等位基因 

分 类 号：R735.7[医药卫生—肿瘤]