高脂饮食对大鼠胰腺腺泡细胞三磷酸肌醇表达及淀粉酶释放影响的体外研究  

In vitro studies of the effects of high-fat dieton the expression of pancreatic acinar cells＇ IP3 and amylase release in rats

作　　者：闫明先[1] 赵华清 王亚茹[1] 李晓荣[3] 杨静[1] 王文奇[1] 王义国[1] 

机构地区：[1]山东大学附属山东省千佛山医院消化内科,济南250014 [2]济南国际机场医疗急救中心 [3]济南市第四人民医院消化内科

出　　处：《中国综合临床》2012年第12期1276-1279,共4页Clinical Medicine of China

基　　金：山东省医药卫生科技发展计划项目（2009HZ069）

摘　　要：目的探讨高脂饮食对胰腺腺泡细胞内三磷酸肌醇（IB）表达及淀粉酶释放的影响。方法雄性Wistar大鼠分为高脂饮食组和正常饮食组，分别喂养4周，全自动生化仪检测血液甘油三酯、胆固醇、淀粉酶和葡萄糖浓度，并观察胰腺病理组织学变化。两组大鼠分离并培养胰腺腺泡细胞，应用[^3H]-IP3检测试剂盒检测细胞内IB浓度；并应用胆囊收缩素（CCK）-8对腺泡细胞刺激培养，检测两组大鼠腺泡细胞淀粉酶释放率。结果高脂饮食大鼠出现高脂血症，其胰腺组织病理染色发现腺泡细胞空泡化，腺泡细胞周围淋巴细胞浸润。高脂饮食大鼠的腺泡细胞IP3含量明显高于正常大鼠[（31．807±3．448）pmol／10^6cells与（24．632±3．649）pmol／10^6cells，t=7．479，P〈0．001]；且高脂饮食大鼠较正常组大鼠的腺泡细胞在不同浓度CCK-8（0．01nmol／L和1nmol／L）刺激下的淀粉酶释放率均明显增高[（11．056±3．369）％与（7．354±2．181）％；t=3．912，P〈0．001；（13．854±4．087）％与（9．432±2．477）％，t=3．939，P〈0．001）。高脂饮食组大鼠的腺泡细胞IP3含量和CCK-8（1nmol／L）刺激下的淀粉酶释放率呈正相关性（r=0．896，P〈0．001）。结论长期高脂血症引起大鼠腺泡细胞对CCK刺激的外分泌敏感性增加，IB作为信号分子具有重要作用。Objective To investigate the effects of high-fat diet on pancreatic acinar cells＇ IP3 expression and CCK-induced amylase release in rats. Methods Male Wistar rats were divided into high-fat diet group and normal diet group, they were fed for 4 weeks. Blood triglycerides, cholesterol, amylase and glucose levelswere determined by automatic biochemical analyzer. Pancreatic tissues were taken for histopathological observations. Pancreatic acinar cells were isolated and cultured, and intracellular inositol 1,4,5-trisphosphate （IP3） was detected using a commercial kit. Amylase release rates were measured after CCK-8 stimulation. Results The rats in high-fat diet group appeared hyperlipidemia, vacuolization of acinar cells and the lymphocytes appeared around the acinar cells can be seen on the pancreatic tissue pathology staining. The levels of IP3 in acinar cells of rats fed a high-fat diet were higher than that of normal rats [ （31. 807 ±3. 448） pmol/10^6cells vs （24. 632±3. 649） pmol/10^6cells, t=7. 479, P 〈 0. 001 ];and amylase release rate in these rats＇ acinar cells were also higher than those of normal rats [ when CCK-8 = 0. 01 nmol/L： （ 11. 056 ±3. 369） % vs （7.354±2. 181）% ,t =3.912,P 〈0.001;when CCK-8 =1 nmol/L： （13. 854 ±4.087）% vs （9.432± 2. 477）% ,t = 3. 939, P 〈 0. 001 ） after CCK-8 stimulation in different concentrations. Additionally, there was a positive co-relationship between acinar cell＇s IP3 level and amylase release （r = 0. 896, P 〈 0. 001 ）. Conclusion Chronic high-fat diet induces hypersensitivity for pancreatic acinar cells＇ exocrine function, and IP3 as a signal molecule may play an important role in this process.

关 键 词：高脂饮食 胰腺腺泡细胞 三磷酸肌醇 外分泌 缩胆囊素 

分 类 号：R576[医药卫生—消化系统]