Oxidative Stress, Biochemical and Histopathological Alterations in the Liver and Kidney of Female Rats Exposed to Low Doses of Deltamethrin (DM):A Molecular Assessment  

作　　者：CHARGUI Issam GRISSA Intissar BENSASSI Fatma HRIRA Mohamed Yahia HAOUEM Samir HAOUAS Zohra BENCHEIKH Hassen 

机构地区：[1]Laboratory of Histology-Cytology and Genetics (02/UR/08-03), Faculty of Medicine, 5019 Monastir, Tunisia [2]Laboratory of Research on Biologically Compatible Compounds, Faculty of Dentistry, Street Avicenne, 5019 Monastir, Tunisia [3]Laboratory of Plant Protection, The National Institute for Agricultural Research, INRA Tunisia, Street Hedi Karray, 2049 Ariana, Tunisia [4]Laboratory of Biochemistry (UR 07/06), Faculty of pharmacy, 5019 Monastir, Tunisia [5]Laboratory of Physiology, Faculty of Medicine, 5019 Monastir, Tunisia

出　　处：《Biomedical and Environmental Sciences》2012年第6期672-683,共12页生物医学与环境科学（英文版）

基　　金：supported by the funds allocated to the Research Unit of Histology & Genetic 02/UR/08-03;the Tunisian Ministry of the Higher Education

摘　　要：Objective To evaluate histopathological alterations of the liver and kidney of female rats exposed to low doses of DM and its potential genotoxic activity. Methods Female Wistar rats were randomly assigned to control （3 groups, 6 rats in each） and treatment groups （3 groups, 6 rats in each）. They were subjected to subcutaneous injections of DM （at doses of 0.003, 0.03, and 0.3 mg/kg bw/d） after 30, 45, and 60 d, respectively. Results Significant alterations were recorded in liver parenchyma induced by hepatic vacuolization, fragmented chromatin in nuclei, dilatation of sinusoids and congestions. Lesions within proximal and distal tubules were observed in the kidneys. Tissue congestions and severe alterations within glomeruli were visible. DM as a pyrethroid insecticide induced significant increase （P〈_O.05） of plasma MDA concentrations after 45 d. A significant increase （P_〈0.05） in plasma ALT （after 45 and 60 d） and AST （after 60 d） concentrations was recorded as compared to controls. During the whole experimental period the toxic agent provoked significant DNA damages （P〈0.05）, especially in the dominance of classes 3 and 4 of obtained comet. Conclusion DM even at a very low dose displays harmful effects by disrupting hepatic and renal function and causing DNA damages in puberscent female rats. Low doses of DM are hepatotoxic and nephrotoxic.Objective To evaluate histopathological alterations of the liver and kidney of female rats exposed to low doses of DM and its potential genotoxic activity. Methods Female Wistar rats were randomly assigned to control （3 groups, 6 rats in each） and treatment groups （3 groups, 6 rats in each）. They were subjected to subcutaneous injections of DM （at doses of 0.003, 0.03, and 0.3 mg/kg bw/d） after 30, 45, and 60 d, respectively. Results Significant alterations were recorded in liver parenchyma induced by hepatic vacuolization, fragmented chromatin in nuclei, dilatation of sinusoids and congestions. Lesions within proximal and distal tubules were observed in the kidneys. Tissue congestions and severe alterations within glomeruli were visible. DM as a pyrethroid insecticide induced significant increase （P〈_O.05） of plasma MDA concentrations after 45 d. A significant increase （P_〈0.05） in plasma ALT （after 45 and 60 d） and AST （after 60 d） concentrations was recorded as compared to controls. During the whole experimental period the toxic agent provoked significant DNA damages （P〈0.05）, especially in the dominance of classes 3 and 4 of obtained comet. Conclusion DM even at a very low dose displays harmful effects by disrupting hepatic and renal function and causing DNA damages in puberscent female rats. Low doses of DM are hepatotoxic and nephrotoxic.

关 键 词：DELTAMETHRIN Rats LIVER KIDNEY HISTOPATHOLOGY Comet assay DNA damage. 

分 类 号：R363[医药卫生—病理学]