芍药花粉提取物的抗氧化能力研究  

Study on Antioxidant Capacity of Peony Pollen Extracts

作　　者：黄海霞[1] 张亚坤[1] 张丹丹[1] 冯蒙[1] 安梦晓[1] 

机构地区：[1]河南科技大学农学院,河南洛阳471003

出　　处：《河南农业科学》2012年第11期117-120,共4页Journal of Henan Agricultural Sciences

基　　金：河南科技大学SRTP项目(No2010182)

摘　　要：为了探究芍药花粉提取液的抗氧化能力和主要活性物质的含量,为开发芍药花粉在天然抗氧化剂和化妆品等相关领域的应用提供一定的理论和试验基础,利用不同体积分数的乙醇溶液制备芍药花粉提取液,测定其主要抗氧化组分黄酮、多酚的含量,通过研究不同质量浓度芍药花粉提取液对DPPH自由基、羟自由基的清除能力,分析芍药花粉提取液的抗氧化效果与其多酚、黄酮含量之间的相关性。结果表明,芍药花粉提取液对DPPH自由基、羟自由基具有一定的清除能力,清除率与提取液质量浓度之间呈现出良好的剂量依赖效应。在高质量浓度(0.8g/L)芍药花粉提取液中,70%乙醇提取液清除DPPH自由基的效果最佳,清除率可达79.88%,而30%乙醇提取液对羟自由基的清除能力最强,清除率达到93.82%。70%乙醇提取液中黄酮、多酚含量均显著高于30%乙醇、100%乙醇提取液中的含量。研究认为,70%乙醇是制备芍药花粉提取液较为理想的溶剂。芍药花粉提取液有着较强的抗氧化能力,其抗氧化性与黄酮含量呈线性正相关,而与多酚含量关系不密切。To explore antioxidant activity of the extracts from peony pollen,and to reveal the ex-perimental and theoretic basis for exploitation of peony pollen in natural antioxidant and cosme-tics applications, the extracts were prepared from peony pollen by different volume fraction of ethanol, and the contents of flavonoids and polyphenols were determined. Antioxidant capacities of different ex-tracts were evaluated by DPPH and hydroxy free radicals elimination methods. Also, the relations between their contents and antioxidant activities were analyzed. The extracts had certain scaven-ging effects on DPPH and hydroxy free radicals,and also the scavenging capability was enhanced with the increase of concentration. Under the high concentration（0.8 g/L）, the 70% ethanol ex-tract gave the best result of elimination of the DPPH free radical, scavenging rate achieving 79.88%. The elimination of the hydroxyl free radical of 30% ethanol extract was the strongest, achieving 93.82%. The contents of flavonoids and polyphenols in 70% ethanol extract were supe-rior to the others obviously. 70% ethanol was ideal solvent to prepare the extract from peony pol-vonoids,but the relation was not close between antioxidant capacities and polyphenols.

关 键 词：芍药 花粉提取液 抗氧化能力 DPPH自由基 羟自由基 

分 类 号：S682.12[农业科学—观赏园艺]