高产γ-氨基丁酸霉菌菌株的筛选及诱变育种  

Screening and Mutation Breeding of a High-Yield γ-Aminobuyric Acid(GABA) Mould Strain

作　　者：边鑫[1] 吴非[1] 

机构地区：[1]东北农业大学食品学院,黑龙江哈尔滨150030

出　　处：《食品科学》2012年第21期213-216,共4页Food Science

基　　金：黑龙江省自然科学基金项目(C200942)

摘　　要：以霉菌作为出发菌株,将其接入大豆-水(3:5,m/V)的发酵培养基中进行发酵,根据发酵过程中γ-氨基丁酸(GABA)产量,筛选出GABA的高产霉菌菌株,然后利用紫外线对高产菌株进行诱变处理,并通过抗性初筛获得长势较好的突变菌株,再分别利用含有质量浓度为1g/100mL L-谷氨酸的PDA综合培养基和大豆水溶液的发酵培养基进行两次筛选,得到稳定高产GABA突变菌株。结果表明,通过对产GABA霉菌菌株的筛选,得到米曲霉3.800为高产GABA霉菌,GABA产量达到0.674g/L。以米曲霉3.800作为原始菌株,对其进行紫外诱变处理,从而获得高产GABA突变菌株,结果表明,利用紫外线对米曲霉3.800进行处理的最佳照射时间为2min。在此照射时间下,通过突变菌株在含有质量浓度为1g/100mL L-谷氨酸的PDA综合培养基和大豆-水溶液的发酵培养基进行两次筛选,最终获得一株稳定的高产GABA突变菌株3.800-4,其在含有质量浓度为1g/100mL谷氨酸的PDA综合培养基中的GABA产量为4.491g/L,比原菌株的GABA产量提高了23.58%;同时其在大豆-水的发酵培养基中的GABA产量为0.874g/L,比原菌株的产量提高了29.67%。In this study,different species of moulds were analyzed for effectiveness in fermenting cooked soybeans suspended in water（3：5,m/V） to produce γ-aminobuyric acid（GABA）.As a result,Aspergillus oryzae 3.800 was found to have the highest GABA producing ability.The strain was mutagenized by UV irradiation for 2 min.For secondary screening,the resulting mutant strains were cultured initially in PDA medium containing 1 g/100 mL L-glutamic acid and then in soybean suspension.A stable high-yield mutant named as 3.800-4 was obtained.The GABA yield of the mutant was 4.491 g/L during culture in PDA medium containing 1 g/100 mL L-glutamic acid and 0.874 g/L during culture in soybean suspension,which was increased by 23.58% and 29.67%,respectively,compared with the original strain.

关 键 词：Γ-氨基丁酸 霉菌 筛选 紫外诱变 高产突变菌株 

分 类 号：TS214.9[轻工技术与工程—粮食、油脂及植物蛋白工程]