高速逆流色谱法分离蜜环菌发酵上清液乙醇提取物条件的研究  

Experimental Conditions for High-Speed Countercurrent Chromatography Separation of Ethanol Extract from Armillaria mellea Fermentation Broth Supernatant

作　　者：蔡丹[1] 刘景圣[1] 郭丽丽[1] 刘义军[1] 陈春阳[1] 

机构地区：[1]吉林农业大学食品科学与工程学院,吉林长春130118

出　　处：《食品科学》2012年第21期245-248,共4页Food Science

基　　金：吉林省科技发展计划项目(20085021)

摘　　要：利用高速逆流色谱法分离蜜环菌发酵液乙醇提取部位,筛选其最佳溶剂体系分离条件。结果表明:溶剂体系为正丁醇-乙酸乙酯-水、且体积比为1:4:5的条件下,能得到最佳分离效果,最终分离出4个峰,效果较好,4个峰的提取率分别为51.10%、1.47%、0.86%、1.80%。This study was designed to investigate experimental conditions for high-speed countercurrent chromatography（HSCCC） separation of the ethanol extract of Armillaria mellea fermentation broth supernatant.The best separation results were achieved using a solvent system composed of butanol,ethyl acetate and deionized water（1：4：5,V/V）.Four peaks were separated well with an extraction yield of 51.10%,1.47%,0.86% and 1.80%,respectively.

关 键 词：蜜环菌发酵液 高速逆流色谱法 溶剂体系 

分 类 号：TS201.3[轻工技术与工程—食品科学]