NJO抑制肿瘤细胞增殖的效力及其作用机制  

The Inhibition and Mechanisms of NJO on Proliferations of Tumor Cells

作　　者：任宏[1] 龙启才[2] 

机构地区：[1]广东食品药品职业学院,广东广州510520 [2]中山大学药学院,广东广州510006

出　　处：《肿瘤基础与临床》2012年第6期467-471,共5页journal of basic and clinical oncology

摘　　要：目的观察邻吡啶醌二取代衍生物(NJO)对4种肿瘤细胞株的体外抑制效力及其作用机制。方法采用噻唑蓝(MTT)和克隆形成法测定NJO对4种肿瘤细胞株(Bel-7402、Glc-82、HT-29、HepG2)的体外抑制效力。同时运用透射电镜、DNA电泳和流式细胞术探讨NJO抑制肿瘤增殖的作用机制。结果 NJO可以明显抑制4种肿瘤细胞的增殖,主要通过诱导肿瘤细胞凋亡的途径抑制细胞增殖。结论 NJO主要通过诱导肿瘤细胞凋亡的途径在体外抑制4种肿瘤细胞株的增殖,且作用存在一定范围的浓度依赖性,是一种有前途的抗肿瘤制剂。Objective To observe the inhibition and mechanisms of m-pyridine-quinone two replace derivatives （NJO） on proliferations of four tumor cell strains in vitro. Methods Methyl thiazolyl tetrazolium（MTT） assay and cloning technique were used to measure the external inhibitive effect of NJO on proliferations of four tumor cell strains （ Bel-7402, Glc-82, HT-29 and HepG2 ）. The mechanisms were analyzed by using the transmission electron microscope observation, DNA electronphoresis and flow cytometric assay. Results NJO could dramatically inhibit the proliferations of four tumor cell strains. NJO inhibited cell proliferations mainly by inducing tumor cell apoptosis. Conclusion NJO can externally inhibit the proliferations of four tumor cell strains by inducing tumor cell apopto- sis, the effects depended on the concentration and the time, it is a promising anti-tumor agent.

关 键 词：邻吡啶醌二取代衍生物 环氧化酶 肿瘤 凋亡 

分 类 号：R730.23[医药卫生—肿瘤] R979.1[医药卫生—临床医学]